Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 25404345
Paz JC, et al. (2014) Combinatorial regulation of a signal-dependent activator by phosphorylation and acetylation. Proc Natl Acad Sci U S A 111, 17116-21 25404345
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S119-p - CREB (human)
Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene
Orthologous residues
CREB (human): S119‑p, CREB iso1 (human): S133‑p, CREB (mouse): S119‑p, CREB iso1 (mouse): S133‑p, CREB (rat): S133‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  '3T3-L1, differentiated' (adipocyte), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
selisistat no change compared to control

K122-ac - CREB (human)
Modsite: sRRPsyRkILNDLss SwissProt Entrez-Gene
Orthologous residues
CREB (human): K122‑ac, CREB iso1 (human): K136‑ac, CREB (mouse): K122‑ac, CREB iso1 (mouse): K136‑ac, CREB (rat): K136‑ac
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE CBP (human) genetic knockout/knockin of upstream enzyme
ACETYLTRANSFERASE p300 (human) genetic knockout/knockin of upstream enzyme, transfection of wild-type enzyme
DEACETYLASE SIRT1 (human) pharmacological inhibitor of upstream enzyme, genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
obesity increase
TNF increase
NKH_477 no change compared to control
trichostatin_A no change compared to control
selisistat increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
p300 (human) Bromodomain Induces NMR spectroscopy, co-immunoprecipitation
CBP (human) Bromodomain Induces NMR spectroscopy, co-immunoprecipitation