Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 25312646
Takii R, et al. (2015) ATF1 Modulates the Heat Shock Response by Regulating the Stress-Inducible Heat Shock Factor 1 Transcription Complex. Mol Cell Biol 35, 11-25 25312646
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S36-p - ATF-1 (mouse)
Modsite: VHQVSSLsESEEsQD SwissProt Entrez-Gene
Orthologous residues
ATF‑1 (human): S36‑p, ATF‑1 (mouse): S36‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell growth, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HSF1 (human) Induces molecular association, regulation
Comments:  HSP70 expression, increases cell survival

S41-p - ATF-1 (mouse)
Modsite: SLsESEEsQDSSDSI SwissProt Entrez-Gene
Orthologous residues
ATF‑1 (human): S41‑p, ATF‑1 (mouse): S41‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell growth, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HSF1 (human) Induces molecular association, regulation
Comments:  HSP70 expression, increases cell survival

S63-p - ATF-1 (mouse)
Modsite: GILARRPsYRKILKD SwissProt Entrez-Gene
Orthologous residues
ATF‑1 (human): S63‑p, ATF‑1 (mouse): S63‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heat_shock increase
MG132 increase
colforsin increase
Downstream Regulation
Effect of modification (function):  acetylation, molecular association, regulation
Effect of modification (process):  cell growth, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Disrupts
Comments:  HSF1 acetylation, Decreased cell survival, disrupts HSF1 binding to HSP70 promoter

S133-p - CREB (mouse)
Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene
Orthologous residues
CREB (human): S133‑p, CREB iso2 (human): S119‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
heat_shock increase
MG132 increase
colforsin increase