Curated Information
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Home > Curated Information Page > PubMed Id: 25329316
Esteve-Puig R, et al. (2014) A Mouse Model Uncovers LKB1 as an UVB-Induced DNA Damage Sensor Mediating CDKN1A (p21WAF1/CIP1) Degradation. PLoS Genet 10, e1004721 25329316
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T80-p - p21Cip1 (human)
Modsite: LPkLYLPtGPRRGRD SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): T80‑p, p21Cip1 (mouse): P79‑p, p21Cip1 (rat): P79‑p, p21Cip1 (dog): A48‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, [32P] bio-synthetic labeling, immunoprecipitation, mass spectrometry (in vitro), western blotting
Disease tissue studied:  BCC
Relevant cell lines - cell types - tissues:  HaCaT (keratinocyte), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (human) microscopy-colocalization, transfection of wild-type enzyme

S146-p - p21Cip1 (human)
Modsite: GRkRRQtsMTDFyHs SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): S146‑p, p21Cip1 (mouse): S141‑p, p21Cip1 (rat): S146‑p, p21Cip1 (dog): S114‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, [32P] bio-synthetic labeling, immunoprecipitation, mass spectrometry (in vitro), western blotting
Disease tissue studied:  BCC
Relevant cell lines - cell types - tissues:  HaCaT (keratinocyte), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE NuaK1 (human)

T366-p - LKB1 (mouse)
Modsite: IEDGIIytQDFTVPG SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): T363‑p, LKB1 iso2 (human): T363‑p, LKB1 (mouse): T366‑p, LKB1 (rat): T366‑p, LKB1 (pig): T271‑p
Downstream Regulation
Effect of modification (function):  protein degradation
Effect of modification (process):  DNA repair, altered

S78-p - p21Cip1 (mouse)
Modsite: GLPKVyLsPGSRSRD SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): P79‑p, p21Cip1 (mouse): S78‑p, p21Cip1 (rat): S78‑p, p21Cip1 (dog): P47‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, [32P] bio-synthetic labeling, immunoprecipitation, mass spectrometry (in vitro), western blotting
Disease tissue studied:  BCC
Relevant cell lines - cell types - tissues:  HaCaT (keratinocyte), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE NuaK1 (human)

S141-p - p21Cip1 (mouse)
Modsite: GRKRRQtsLTDFYHS SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): S146‑p, p21Cip1 (mouse): S141‑p, p21Cip1 (rat): S146‑p, p21Cip1 (dog): S114‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, [32P] bio-synthetic labeling, immunoprecipitation, mass spectrometry (in vitro), western blotting
Disease tissue studied:  BCC
Relevant cell lines - cell types - tissues:  HaCaT (keratinocyte), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE NuaK1 (human)