Chaudhary N, et al. (2014) SMAR1 coordinates HDAC6-induced deacetylation of Ku70 and dictates cell fate upon irradiation. Cell Death Dis 5, e1447 25299772

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S1981-p - ATM (human) ▲

Modsite: sLAFEEGsQSTtIss SwissProt Entrez-Gene Orthologous residues ATM (human): S1981‑p, ATM (mouse): S1987‑p, ATM (rat): S1988‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ionizing_radiation increase KU-55933 ionizing_radiation inhibit treatment-induced increase ionizing_radiation increase KU-55933 ionizing_radiation inhibit treatment-induced increase Downstream Regulation Effect of modification (process):  cell cycle regulation, DNA repair, induced

S216-p - CDC25C (human) ▲
Modsite: sGLyRsPsMPENLNR SwissProt Entrez-Gene Orthologous residues CDC25C (human): S216‑p, CDC25C iso3 (human): S173‑p, CDC25C (mouse): ‑, CDC25C (frog): S287‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human

Y15-p - CDK1 (human) ▲
Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene Orthologous residues CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human

T68-p - Chk2 (human) ▲

Modsite: SsLEtVstQELYsIP SwissProt Entrez-Gene Orthologous residues Chk2 (human): T68‑p, Chk2 iso9 (human): T68‑p, Chk2 iso11 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (process):  cell cycle regulation, DNA repair, induced

S10-p - H3 (human) ▲

Modsite: tkQtArkstGGkAPr SwissProt Entrez-Gene Orthologous residues H3 (human): S10‑p, H3 (mouse): S10‑p, H3 iso2 (mouse): S10‑p, H3 iso3 (mouse): S10‑p, H3 (rat): S10‑p, H3 iso3 (rat): S10‑p, H3 (pig): S10‑p, H3 (chicken): S10‑p, H3 (cow): S10‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human

S370-p - BANP (mouse) ▲

Modsite: PTSELQQsQPQALHY SwissProt Entrez-Gene Orthologous residues BANP (human): P362‑p, BANP iso2 (human): P331‑p, BANP (mouse): S370‑p, BANP (rat): S370‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  ataxia-telangiectasia, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  C3ABR (lymphoblastoid), HCT116 (intestinal), L3 (lymphoblastoid), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE ATM (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ATM (human) pharmacological inhibitor of upstream enzyme, modification site within consensus motif, activation of upstream enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ionizing_radiation increase KU-55933 ionizing_radiation inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Effect of modification (process):  apoptosis, inhibited, cell cycle regulation, DNA repair, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays H2AX (mouse) Induces co-immunoprecipitation, microscopy-colocalization Comments:  radioresistance imparted to cells, regulates interaction between Ku70 and Bax