Curated Information
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Home > Curated Information Page > PubMed Id: 25315833
Zhou GL, et al. (2014) Phosphorylation of the cytoskeletal protein CAP1 controls its association with cofilin and actin. J Cell Sci 127, 5052-65 25315833
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S36-p - CAP1 (mouse)
Modsite: CGyGDsPsKGAVPYV SwissProt Entrez-Gene
Orthologous residues
CAP1 (human): S36‑p, CAP1 iso2 (human): S36‑p, CAP1 (mouse): S36‑p, CAP1 (rat): S36‑p
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell motility, inhibited, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Cofilin-1 (human) Induces pull-down assay

S307-p - CAP1 (mouse)
Modsite: SAPKPQtsPsPKPAt SwissProt Entrez-Gene
Orthologous residues
CAP1 (human): S308‑p, CAP1 iso2 (human): S307‑p, CAP1 (mouse): S307‑p, CAP1 (rat): S307‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
colforsin decrease
SB216763 decrease
lithium decrease
BIO decrease
LY294002 increase
U0126 no change compared to control
RKI-1447 no change compared to control
siRNA decrease GSK3B siRNA
fibronectin decrease
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell motility, inhibited, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ACTA1 (human) Induces co-immunoprecipitation
Cofilin-1 (human) Disrupts pull-down assay

S309-p - CAP1 (mouse)
Modsite: PKPQtsPsPKPAtKK SwissProt Entrez-Gene
Orthologous residues
CAP1 (human): S310‑p, CAP1 iso2 (human): S309‑p, CAP1 (mouse): S309‑p, CAP1 (rat): S309‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, [32P] bio-synthetic labeling, immunoassay, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), HEK293T (epithelial), HeLa (cervical), hTERT-HPNE (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) modification site within consensus motif, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell motility, inhibited, cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Cofilin-1 (human) Disrupts pull-down assay
ACTA1 (human) Induces co-immunoprecipitation