Curated Information
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Home > Curated Information Page > PubMed Id: 25192600
Yan J, et al. (2015) Blockage of GSK3β-mediated Drp1 phosphorylation provides neuroprotection in neuronal and mouse models of Alzheimer's disease. Neurobiol Aging 36, 211-27 25192600
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S40-p - DRP1 (human)
Modsite: TQSSGKSsVLEsLVG SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): S40‑p, DRP1 iso2 (human): S40‑p, DRP1 iso3 (human): S40‑p, DRP1 iso4 (human): S40‑p, DRP1 iso6 (human): S40‑p, DRP1 (mouse): S40‑p, DRP1 iso3 (mouse): S40‑p, DRP1 iso6 (mouse): S40‑p, DRP1 (rat): S40‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  293 (epithelial), neuron-'brain, hippocampus'
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) co-immunoprecipitation, transfection of wild-type enzyme, phospho-antibody
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization
Effect of modification (process):  apoptosis, induced
Comments:  mitochondrial localization, increased GTPase activity, mitochondrial fragmentation
Associated Diseases
Diseases Alterations Comments
Alzheimer's disease increased blocking phosphorylation is neuroprotective in models

S44-p - DRP1 (human)
Modsite: GKSsVLEsLVGRDLL SwissProt Entrez-Gene
Orthologous residues
DRP1 (human): S44‑p, DRP1 iso2 (human): S44‑p, DRP1 iso3 (human): S44‑p, DRP1 iso4 (human): S44‑p, DRP1 iso6 (human): S44‑p, DRP1 (mouse): S44‑p, DRP1 iso3 (mouse): S44‑p, DRP1 iso6 (mouse): S44‑p, DRP1 (rat): S44‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  293 (epithelial), neuron-'brain, hippocampus'
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE GSK3B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) co-immunoprecipitation, transfection of wild-type enzyme, phospho-antibody
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, phosphorylation
Effect of modification (process):  apoptosis, induced
Comments:  mitochondrial localization, increased GTPase activity, mitochondrial fragmentation, S44 serves as a priming site for S40 phosphorylation
Associated Diseases
Diseases Alterations Comments
Alzheimer's disease increased blocking phosphorylation is neuroprotective in models