Kang M, et al. (2014) Cx43 phosphorylation on S279/282 and intercellular communication are regulated by IP3/IP3 receptor signaling. Cell Commun Signal 12, 58 25262337

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S262-p - GJA1 (mouse) ▲

Modsite: sPskDCGsPkyAyFN SwissProt Entrez-Gene Orthologous residues GJA1 (human): S262‑p, GJA1 (mouse): S262‑p, GJA1 (rat): S262‑p, GJA1 (rabbit): S262‑p, GJA1 (pig): S262‑p, GJA1 (hamster): S262‑p, GJA1 (cow): S263‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'heart, ventricle', myocyte-heart Cellular systems studied:  primary cultured cells, tissue Species studied:  mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes 2-APB decrease ATP increase XeC increase

S368-p - GJA1 (mouse) ▲

Modsite: QRPssRAssRAssRP SwissProt Entrez-Gene Orthologous residues GJA1 (human): S368‑p, GJA1 (mouse): S368‑p, GJA1 (rat): S368‑p, GJA1 (rabbit): S368‑p, GJA1 (pig): S368‑p, GJA1 (hamster): S368‑p, GJA1 (cow): S369‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'heart, ventricle', myocyte-heart Cellular systems studied:  primary cultured cells, tissue Species studied:  mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes 2-APB decrease ATP increase XeC increase

S262-p - GJA1 (rat) ▲

Modsite: sPskDCGsPkyAyFN SwissProt Entrez-Gene Orthologous residues GJA1 (human): S262‑p, GJA1 (mouse): S262‑p, GJA1 (rat): S262‑p, GJA1 (rabbit): S262‑p, GJA1 (pig): S262‑p, GJA1 (hamster): S262‑p, GJA1 (cow): S263‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'heart, ventricle', myocyte-heart Cellular systems studied:  primary cultured cells, tissue Species studied:  mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes 2-APB decrease IP3R1 (rat) increase shRNA decrease

S279-p - GJA1 (rat) ▲

Modsite: SSPTAPLsPMsPPGY SwissProt Entrez-Gene Orthologous residues GJA1 (human): S279‑p, GJA1 (mouse): S279‑p, GJA1 (rat): S279‑p, GJA1 (rabbit): S279‑p, GJA1 (pig): S279‑p, GJA1 (hamster): S279‑p, GJA1 (cow): S280‑p Characterization Methods used to characterize site in vivo:  immunoassay, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'heart, ventricle', myocyte-heart Cellular systems studied:  cell lines, primary cultured cells, tissue Species studied:  human, mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IP3/BM increase ATP increase IP3R1 (rat) increase shRNA decrease 2-APB decrease phorbol_ester increase Downstream Regulation Comments:  regulates gap junction permeability and intercellular communication

S282-p - GJA1 (rat) ▲

Modsite: TAPLsPMsPPGYkLV SwissProt Entrez-Gene Orthologous residues GJA1 (human): S282‑p, GJA1 (mouse): S282‑p, GJA1 (rat): S282‑p, GJA1 (rabbit): S282‑p, GJA1 (pig): S282‑p, GJA1 (hamster): S282‑p, GJA1 (cow): S283‑p Characterization Methods used to characterize site in vivo:  immunoassay, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'heart, ventricle', myocyte-heart Cellular systems studied:  cell lines, primary cultured cells, tissue Species studied:  human, mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes IP3/BM increase ATP increase IP3R1 (rat) increase shRNA decrease 2-APB decrease phorbol_ester increase Downstream Regulation Comments:  regulates gap junction permeability and intercellular communication

S368-p - GJA1 (rat) ▲

Modsite: QRPssRAssRAssRP SwissProt Entrez-Gene Orthologous residues GJA1 (human): S368‑p, GJA1 (mouse): S368‑p, GJA1 (rat): S368‑p, GJA1 (rabbit): S368‑p, GJA1 (pig): S368‑p, GJA1 (hamster): S368‑p, GJA1 (cow): S369‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'heart, ventricle', myocyte-heart Cellular systems studied:  primary cultured cells, tissue Species studied:  mouse, rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase 2-APB decrease IP3R1 (rat) increase IP3R1-shRNA decreases