Curated Information
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Home > Curated Information Page > PubMed Id: 25204653
Crowell S, et al. (2014) Post-translational Regulation of Mitogen-activated Protein Kinase Phosphatase (MKP)-1 and MKP-2 in Macrophages Following Lipopolysaccharide Stimulation: THE ROLE OF THE C TERMINI OF THE PHOSPHATASES IN DETERMINING THEIR STABILITY. J Biol Chem 289, 28753-64 25204653
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S359-p - DUSP1 (human)
Modsite: SALSYLQsPITTsPS SwissProt Entrez-Gene
Orthologous residues
DUSP1 (human): S359‑p, DUSP1 (mouse): S359‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), RAW 264.7 (macrophage)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
U0126 LPS inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  protein stabilization

S364-p - DUSP1 (human)
Modsite: LQsPITTsPSC____ SwissProt Entrez-Gene
Orthologous residues
DUSP1 (human): S364‑p, DUSP1 (mouse): S364‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), RAW 264.7 (macrophage)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
U0126 LPS inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  protein stabilization

S386-p - DUSP4 (human)
Modsite: SSLPYLHsPITTsPS SwissProt Entrez-Gene
Orthologous residues
DUSP4 (human): S386‑p, DUSP4 iso2 (human): S295‑p, DUSP4 (mouse): S390‑p, DUSP4 (rat): S387‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), RAW 264.7 (macrophage)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
U0126 LPS inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  protein stabilization

S391-p - DUSP4 (human)
Modsite: LHsPITTsPSC____ SwissProt Entrez-Gene
Orthologous residues
DUSP4 (human): S391‑p, DUSP4 iso2 (human): S300‑p, DUSP4 (mouse): S395‑p, DUSP4 (rat): S392‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), RAW 264.7 (macrophage)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
U0126 LPS inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  protein stabilization