Curated Information
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Home > Curated Information Page > PubMed Id: 15024053
Storz P, Döppler H, Toker A (2004) Protein kinase Cdelta selectively regulates protein kinase D-dependent activation of NF-kappaB in oxidative stress signaling. Mol Cell Biol 24, 2614-26 15024053
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T507-p - PKCD (human)
Modsite: FGEsRAstFCGtPDy SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

Y463-p - PRKD1 (human)
Modsite: NDTGsRYyKEIPLSE SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): Y463‑p, PRKD1 (mouse): Y469‑p, PRKD1 (rat): Y469‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation, protein conformation
Effect of modification (process):  transcription, altered
Comments:  activates transciption, constitutively active Y463E mutant stimulates phosphorylation of residues S738/S742

S738-p - PRKD1 (human)
Modsite: ARIIGEksFRRsVVG SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S738‑p, PRKD1 (mouse): S744‑p, PRKD1 (rat): S744‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCE (human)
KINASE PKCD (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (human) transfection of dominant-negative enzyme, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
H2O2 PKCD (human) inhibit treatment-induced increase PKCD siRNA
vanadate increase
phorbol_ester increase
BSO increase
Src (human) increase Src Y527F
Abl (human) increase v-Abl
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  transcription, altered
Comments:  activates transciption

S742-p - PRKD1 (human)
Modsite: GEksFRRsVVGtPAy SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S742‑p, PRKD1 (mouse): S748‑p, PRKD1 (rat): S748‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCD (human)
KINASE PKCE (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (human) transfection of dominant-negative enzyme, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
H2O2 PKCD (human) inhibit treatment-induced increase PKCD siRNA
vanadate increase
phorbol_ester increase
BSO increase
Src (human) increase Src Y527F
Abl (human) increase v-Abl
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  transcription, altered
Comments:  activates transciption