Widmann C, Dolci W, Thorens B (1996) Heterologous desensitization of the glucagon-like peptide-1 receptor by phorbol esters requires phosphorylation of the cytoplasmic tail at four different sites. J Biol Chem 271, 19957-63 8702711

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S431-p - GLP1R (rat) ▲

Modsite: RLNIQRDssMKPLKC SwissProt Entrez-Gene Orthologous residues GLP1R (human): S431‑p, GLP1R (mouse): C431‑p, GLP1R (rat): S431‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  CHL (fibroblast), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCA (rat) Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DiC8 increase phorbol_ester increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered

S432-p - GLP1R (rat) ▲

Modsite: LNIQRDssMKPLKCP SwissProt Entrez-Gene Orthologous residues GLP1R (human): S432‑p, GLP1R (mouse): S432‑p, GLP1R (rat): S432‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  CHL (fibroblast), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCA (rat) Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase DiC8 increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered

S441-p - GLP1R (rat) ▲

Modsite: KPLKCPTssVssGAT SwissProt Entrez-Gene Orthologous residues GLP1R (human): S441‑p, GLP1R (mouse): S441‑p, GLP1R (rat): S441‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  CHL (fibroblast), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DiC8 increase phorbol_ester increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered

S442-p - GLP1R (rat) ▲

Modsite: PLKCPTssVssGATV SwissProt Entrez-Gene Orthologous residues GLP1R (human): S442‑p, GLP1R (mouse): S442‑p, GLP1R (rat): S442‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  CHL (fibroblast), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase DiC8 increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered

S444-p - GLP1R (rat) ▲

Modsite: KCPTssVssGATVGs SwissProt Entrez-Gene Orthologous residues GLP1R (human): S444‑p, GLP1R (mouse): S444‑p, GLP1R (rat): S444‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  CHL (fibroblast), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase DiC8 increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered

S445-p - GLP1R (rat) ▲

Modsite: CPTssVssGATVGss SwissProt Entrez-Gene Orthologous residues GLP1R (human): S445‑p, GLP1R (mouse): S445‑p, GLP1R (rat): S445‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  CHL (fibroblast), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase DiC8 increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered

S451-p - GLP1R (rat) ▲

Modsite: ssGATVGssVYAATC SwissProt Entrez-Gene Orthologous residues GLP1R (human): S451‑p, GLP1R (mouse): S451‑p, GLP1R (rat): S451‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  CHL (fibroblast), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase DiC8 increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered

S452-p - GLP1R (rat) ▲

Modsite: sGATVGssVYAATCQ SwissProt Entrez-Gene Orthologous residues GLP1R (human): S452‑p, GLP1R (mouse): S452‑p, GLP1R (rat): S452‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis Relevant cell lines - cell types - tissues:  CHL (fibroblast), COS (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase DiC8 increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered