Curated Information
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Home > Curated Information Page > PubMed Id: 15096610
Lin SY, Li K, Stewart GS, Elledge SJ (2004) Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation. Proc Natl Acad Sci U S A 101, 6484-9 15096610
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S1524-p - BRCA1 (human)
Modsite: LQNRNYPsQEELIKV SwissProt Entrez-Gene
Orthologous residues
BRCA1 (human): S1524‑p, BRCA1 iso6 (human): S420‑p, BRCA1 iso7 (human): S1545‑p, BRCA1 (mouse): S1481‑p, BRCA1 (rat): S1482‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  HeLa (cervical), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing_radiation increase
siRNA UV claspin (human) inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
claspin (human) Induces cell cycle regulation co-immunoprecipitation

S345-p - Chk1 (human)
Modsite: LVQGIsFsQPtCPDH SwissProt Entrez-Gene
Orthologous residues
Chk1 (human): S345‑p, Chk1 (mouse): S345‑p, Chk1 (rat): S345‑p, Chk1 (chicken): S345‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing_radiation increase
siRNA UV claspin (human) inhibit treatment-induced increase

T68-p - Chk2 (human)
Modsite: SsLEtVstQELYsIP SwissProt Entrez-Gene
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso9 (human): T68‑p, Chk2 iso11 (human): , Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV increase
ionizing_radiation increase
siRNA UV claspin (human) inhibit treatment-induced increase