Curated Information
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Home > Curated Information Page > PubMed Id: 25119572
Kanthou C, et al. (2014) Tumour cells expressing single VEGF isoforms display distinct growth, survival and migration characteristics. PLoS One 9, e104015 25119572
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y1333-p - VEGFR1 (human)
Modsite: DyNSVVLySTPPI__ SwissProt Entrez-Gene
Orthologous residues
VEGFR1 (human): Y1333‑p, VEGFR1 iso2 (human): , VEGFR1 iso8 (human): Y356‑p, VEGFR1 (mouse): Y1328‑p, VEGFR1 (rat): Y1331‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines

Y996-p - VEGFR2 (human)
Modsite: EEAPEDLyKDFLTLE SwissProt Entrez-Gene
Orthologous residues
VEGFR2 (human): Y996‑p, VEGFR2 (mouse): Y994‑p, VEGFR2 (rat): Y992‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines

Y1175-p - VEGFR2 (human)
Modsite: AQQDGKDyIVLPISE SwissProt Entrez-Gene
Orthologous residues
VEGFR2 (human): Y1175‑p, VEGFR2 (mouse): Y1173‑p, VEGFR2 (rat): Y1171‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PIK3C2A (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF (human) increase in fswt & fs188 cells
JAK_inhibitor_I VEGF (human) inhibit treatment-induced increase p6 jak inhibitor
LY294002 VEGF (human) inhibit treatment-induced increase

S253-p - FOXO1A (mouse)
Modsite: sPRRRAAsMDNNSKF SwissProt Entrez-Gene
Orthologous residues
FOXO1A (human): S256‑p, FOXO1A (mouse): S253‑p, FOXO1A (rat): S250‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF (human) increase in fs164 & fs120 cells
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  signaling pathway regulation, transcription, inhibited

T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PIK3C2A (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF (human) increase in fswt & fs188 cells
JAK_inhibitor_I VEGF (human) inhibit treatment-induced increase p6 jak inhibitor
LY294002 VEGF (human) inhibit treatment-induced increase

Y705-p - STAT3 (mouse)
Modsite: DPGsAAPyLktkFIC SwissProt Entrez-Gene
Orthologous residues
STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 iso3 (human): Y705‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK1 (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
VEGF (human) increase in fswt & fs188 cells
JAK_inhibitor_I VEGF (human) inhibit treatment-induced increase p6 jak inhibitor
LY294002 VEGF (human) inhibit treatment-induced increase

Y994-p - VEGFR2 (mouse)
Modsite: EEASEELyKDFLTLE SwissProt Entrez-Gene
Orthologous residues
VEGFR2 (human): Y996‑p, VEGFR2 (mouse): Y994‑p, VEGFR2 (rat): Y992‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines

Y1173-p - VEGFR2 (mouse)
Modsite: AQQDGKDyIVLPMSE SwissProt Entrez-Gene
Orthologous residues
VEGFR2 (human): Y1175‑p, VEGFR2 (mouse): Y1173‑p, VEGFR2 (rat): Y1171‑p
Characterization
Methods used to characterize site in vivo western blotting
Cellular systems studied:  cell lines
Species studied:  human
Comments:  fibrosarcoma cell lines