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Home > Curated Information Page > PubMed Id: 25090023
Das JK, Felty Q (2014) PCB153-Induced Overexpression of ID3 Contributes to the Development of Microvascular Lesions. PLoS One 9, e104159 25090023
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S5-p - ID3 (human)
Modsite: ___MkALsPVRGCyE SwissProt Entrez-Gene
Orthologous residues
ID3 (human): S5‑p, ID3 (mouse): S5‑p, ID3 (rat): S5‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  EC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pyk2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  protein stabilization
Comments:  regulates vascular EC survival and growth of microvascular lesions

Y11-p - ID3 (human)
Modsite: LsPVRGCyEAVCCLs SwissProt Entrez-Gene
Orthologous residues
ID3 (human): Y11‑p, ID3 (mouse): Y11‑p, ID3 (rat): Y11‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  EC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pyk2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  protein stabilization
Comments:  regulates vascular EC survival and growth of microvascular lesions

S18-p - ID3 (human)
Modsite: yEAVCCLsERsLAIA SwissProt Entrez-Gene
Orthologous residues
ID3 (human): S18‑p, ID3 (mouse): S18‑p, ID3 (rat): S18‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  EC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  protein stabilization
Comments:  regulates vascular EC survival and growth of microvascular lesions

Y48-p - ID3 (human)
Modsite: LDDMNHCySRLRELV SwissProt Entrez-Gene
Orthologous residues
ID3 (human): Y48‑p, ID3 (mouse): Y48‑p, ID3 (rat): Y48‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  EC (endothelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pyk2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  protein stabilization
Comments:  regulates vascular EC survival and growth of microvascular lesions

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NAC decrease
PF-431396 decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced