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Home > Curated Information Page > PubMed Id: 24981431
Zhao C, et al. (2015) The Upregulation of NR2A-Containing N-Methyl-D-Aspartate Receptor Function by Tyrosine Phosphorylation of Postsynaptic Density 95 Via Facilitating Src/Proline-Rich Tyrosine Kinase 2 Activation. Mol Neurobiol 51, 500-11 24981431
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y523-p - PSD-95 (human)
Modsite: REDSVLSyETVTQME SwissProt Entrez-Gene
Orthologous residues
PSD‑95 (human): Y523‑p, PSD‑95 iso2 (human): Y566‑p, PSD‑95 (mouse): Y523‑p, PSD‑95 (rat): Y523‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HEK293T (epithelial)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) co-immunoprecipitation, transfection of inactive enzyme, transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NMDA increase
bicuculline increase
MK801 bicuculline, NMDA inhibit treatment-induced increase
PP2 bicuculline, NMDA inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  activity, induced, molecular association, regulation, phosphorylation
Effect of modification (process):  neural plasticity
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Pyk2 (human) Induces co-immunoprecipitation
Comments:  activation of NMDA receptor function, NR2A phosphorylation

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HEK293T (epithelial)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NMDA increase
NMDA PSD-95 (human) inhibit treatment-induced increase Y523F mutant

Y419-p - Src (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HEK293T (epithelial)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NMDA increase
NMDA PSD-95 (human) inhibit treatment-induced increase Y523F mutant