Curated Information
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Home > Curated Information Page > PubMed Id: 11909964
Shiraha H, et al. (2002) Activation of m-calpain (calpain II) by epidermal growth factor is limited by protein kinase A phosphorylation of m-calpain. Mol Cell Biol 22, 2716-27 11909964
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S369-p - CAPN2 (human)
Modsite: DGNWRRGstAGGCRN SwissProt Entrez-Gene
Orthologous residues
CAPN2 (human): S369‑p, CAPN2 iso2 (human): S291‑p, CAPN2 (mouse): S369‑p, CAPN2 (rat): S369‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  NR6WT (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cAMP_analog increase
H-89 cAMP_analog inhibit treatment-induced increase
8-Rp-cAMP cAMP_analog inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  cell motility, altered

T370-p - CAPN2 (human)
Modsite: GNWRRGstAGGCRNY SwissProt Entrez-Gene
Orthologous residues
CAPN2 (human): T370‑p, CAPN2 iso2 (human): T292‑p, CAPN2 (mouse): T370‑p, CAPN2 (rat): T370‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  NR6WT (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cAMP_analog increase
H-89 cAMP_analog inhibit treatment-induced increase
8-Rp-cAMP cAMP_analog inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  cell motility, altered