Curated Information
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Home > Curated Information Page > PubMed Id: 19448664
Liao Y, et al. (2009) Peptidyl-prolyl cis/trans isomerase Pin1 is critical for the regulation of PKB/Akt stability and activation phosphorylation. Oncogene 28, 2436-45 19448664
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T92-p - Akt1 (human)
Modsite: ERtFHVEtPEEREEW SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T92‑p, Akt1 iso2 (human): T30‑p, Akt1 (mouse): T92‑p, Akt1 (rat): T92‑p, Akt1 (fruit fly): S195‑p, Akt1 (cow): T92‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation, protein conformation, protein stabilization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIN1 (human) Induces co-immunoprecipitation, pull-down assay
Comments:  sites can increase Akt activation slightly and maintain activation and stability

T450-p - Akt1 (human)
Modsite: tAQMItItPPDQDDs SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T450‑p, Akt1 iso2 (human): T388‑p, Akt1 (mouse): T450‑p, Akt1 (rat): T450‑p, Akt1 (fruit fly): T565‑p, Akt1 (cow): T450‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation, protein conformation, protein stabilization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PIN1 (human) Induces co-immunoprecipitation, pull-down assay
Comments:  sites can increase Akt activation slightly and maintain activation and stability

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  breast cancer, breast adenocarcinoma, breast cancer, triple negative
Relevant cell lines - cell types - tissues:  MDA-MB-468 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
LY294002 IGF-1 inhibit treatment-induced increase