Garg P, et al. (2007) Neph1 cooperates with nephrin to transduce a signal that induces actin polymerization. Mol Cell Biol 27, 8698-712 17923684

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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Y604-p - KIRREL (mouse) ▲

Modsite: TRVMKAIysSFKDDV SwissProt Entrez-Gene Orthologous residues KIRREL (human): Y572‑p, KIRREL iso2 (human): Y588‑p, KIRREL (mouse): Y604‑p, KIRREL (rat): Y604‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mutation of modification site, peptide sequencing, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS7 (fibroblast), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  bacteria, green monkey, rat

Y637-p - KIRREL (mouse) ▲

Modsite: MkDPTNGyyNVRAHE SwissProt Entrez-Gene Orthologous residues KIRREL (human): Y605‑p, KIRREL iso2 (human): Y621‑p, KIRREL (mouse): Y637‑p, KIRREL (rat): Y637‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoassay, immunoprecipitation, mutation of modification site, peptide sequencing, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  bacteria, green monkey, mouse, rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE Fyn (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fyn (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of constitutively active enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays GRB2 (mouse) SH2 Induces cytoskeletal reorganization pull-down assay, co-immunoprecipitation, microscopy-colocalization

Y638-p - KIRREL (mouse) ▲

Modsite: kDPTNGyyNVRAHED SwissProt Entrez-Gene Orthologous residues KIRREL (human): Y606‑p, KIRREL iso2 (human): Y622‑p, KIRREL (mouse): Y638‑p, KIRREL (rat): Y638‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoassay, immunoprecipitation, mutation of modification site, peptide sequencing, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  bacteria, green monkey, mouse, rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE Fyn (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fyn (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of constitutively active enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays GRB2 (mouse) SH2 Induces cytoskeletal reorganization pull-down assay, co-immunoprecipitation, microscopy-colocalization

Y716-p - KIRREL (mouse) ▲

Modsite: DTTSQLSyENyEKFN SwissProt Entrez-Gene Orthologous residues KIRREL (human): Y684‑p, KIRREL iso2 (human): Y700‑p, KIRREL (mouse): Y716‑p, KIRREL (rat): Y716‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mutation of modification site, peptide sequencing, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS7 (fibroblast), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  bacteria, green monkey, rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE Fyn (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fyn (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of constitutively active enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme

Y719-p - KIRREL (mouse) ▲

Modsite: SQLSyENyEKFNSHP SwissProt Entrez-Gene Orthologous residues KIRREL (human): Y687‑p, KIRREL iso2 (human): Y703‑p, KIRREL (mouse): Y719‑p, KIRREL (rat): Y719‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mutation of modification site, peptide sequencing, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS7 (fibroblast), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  bacteria, green monkey, rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE Fyn (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Fyn (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of constitutively active enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme