Curated Information
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Home > Curated Information Page > PubMed Id: 18156174
Kang S, et al. (2008) Epidermal growth factor stimulates RSK2 activation through activation of the MEK/ERK pathway and src-dependent tyrosine phosphorylation of RSK2 at Tyr-529. J Biol Chem 283, 4652-7 18156174
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S386-p - RSK2 (human)
Modsite: HQLFRGFsFVAItsD SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): S386‑p, RSK2 (mouse): S386‑p, RSK2 (rat): S386‑p, RSK2 (cow): S386‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
U0126 EGF inhibit treatment-induced increase
PP2 EGF inhibit treatment-induced increase

Y470-p - RSK2 (human)
Modsite: EIEILLRyGQHPNII SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): Y470‑p, RSK2 (mouse): Y470‑p, RSK2 (rat): Y470‑p, RSK2 (cow): Y470‑p

Y483-p - RSK2 (human)
Modsite: IItLkDVyDDGKyVY SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): Y483‑p, RSK2 (mouse): Y483‑p, RSK2 (rat): Y483‑p, RSK2 (cow): Y483‑p

Y488-p - RSK2 (human)
Modsite: DVyDDGKyVYVVTEL SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): Y488‑p, RSK2 (mouse): Y488‑p, RSK2 (rat): Y488‑p, RSK2 (cow): Y488‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)
KINASE Src (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) phospho-motif antibody, modification site within consensus motif, transfection of constitutively active enzyme

Y529-p - RSK2 (human)
Modsite: TITKTVEyLHAQGVV SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): Y529‑p, RSK2 (mouse): Y529‑p, RSK2 (rat): Y529‑p, RSK2 (cow): Y529‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293T (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)
KINASE FGFR3 (human)
KINASE Src (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Fyn (human) phospho-antibody, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, transfection of constitutively active enzyme
KINASE Src (human) phospho-antibody, pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
U0126 EGF no effect upon treatment-induced increase
PP2 EGF inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ERK1 (human) Induces co-immunoprecipitation
ERK2 (human) Induces co-immunoprecipitation

Y580-p - RSK2 (human)
Modsite: GLLMtPCytANFVAP SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): Y580‑p, RSK2 (mouse): Y580‑p, RSK2 (rat): Y580‑p, RSK2 (cow): Y580‑p

Y644-p - RSK2 (human)
Modsite: KFSLSGGyWNSVSDT SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): Y644‑p, RSK2 (mouse): Y644‑p, RSK2 (rat): Y644‑p, RSK2 (cow): Y644‑p

Y707-p - RSK2 (human)
Modsite: KGAMAAtysALNRNQ SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): Y707‑p, RSK2 (mouse): Y707‑p, RSK2 (rat): Y707‑p, RSK2 (cow): Y707‑p