Choi SH, et al. (2013) α-Catenin interacts with APC to regulate β-catenin proteolysis and transcriptional repression of Wnt target genes. Genes Dev 27, 2473-88 24240237

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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Y177-p - CTNNA1 (human) ▲

Modsite: EQDLGIQykALkPEV SwissProt Entrez-Gene Orthologous residues CTNNA1 (human): Y177‑p, CTNNA1 iso2 (human): Y177‑p, CTNNA1 (mouse): Y177‑p, CTNNA1 (rat): Y177‑p, CTNNA1 (cow): Y177‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes dasatinib decrease Downstream Regulation Effect of modification (function):  activity, induced, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays APC (human) Disrupts co-immunoprecipitation Comments:  enhances B-catenin transactivation

S33-p - CTNNB1 (human) ▲

Modsite: QQQsyLDsGIHsGAT SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p, CTNNB1 (rabbit): S33‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CTNNA1 (human) increase siRNA decrease APC (human) increase siRNA decrease

T120-p - CTNNB1 (human) ▲

Modsite: QFdAAHPtNVQRLAE SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): T120‑p, CTNNB1 (mouse): T120‑p, CTNNB1 (rat): T120‑p, CTNNB1 (rabbit): T120‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CTNNA1 (human) Induces co-immunoprecipitation

Y142-p - CTNNB1 (human) ▲

Modsite: AVVNLINyQDDAELA SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): Y142‑p, CTNNB1 (mouse): Y142‑p, CTNNB1 (rat): Y142‑p, CTNNB1 (rabbit): Y142‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CTNNA1 (human) Induces co-immunoprecipitation