Curated Information
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Home > Curated Information Page > PubMed Id: 25002582
Bhatnagar S, et al. (2014) Phosphorylation and Degradation of Tomosyn-2 De-represses Insulin Secretion. J Biol Chem 289, 25276-86 25002582
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S763-p - STXBP5L (human)
Modsite: CTSPTSQsCSsGKRL SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S763‑p, STXBP5L (mouse): S762‑p, STXBP5L iso8 (mouse): S769‑p, STXBP5L (rat): S762‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;

S766-p - STXBP5L (human)
Modsite: PTSQsCSsGKRLssA SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S766‑p, STXBP5L (mouse): S765‑p, STXBP5L iso8 (mouse): S772‑p, STXBP5L (rat): S765‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;

S771-p - STXBP5L (human)
Modsite: CSsGKRLssADVSKV SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S771‑p, STXBP5L (mouse): S770‑p, STXBP5L iso8 (mouse): S777‑p, STXBP5L (rat): S770‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cAMP_analog increase
glucose increase
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;

S772-p - STXBP5L (human)
Modsite: SsGKRLssADVSKVN SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S772‑p, STXBP5L (mouse): S771‑p, STXBP5L iso8 (mouse): S778‑p, STXBP5L (rat): S771‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cAMP_analog increase
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;

S800-p - STXBP5L (human)
Modsite: sAACMEIsLPVTTEE SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S800‑p, STXBP5L (mouse): S799‑p, STXBP5L iso8 (mouse): S806‑p, STXBP5L (rat): S799‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;

S812-p - STXBP5L (human)
Modsite: TEENRENsYNRsRsS SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S812‑p, STXBP5L (mouse): S811‑p, STXBP5L iso8 (mouse): S818‑p, STXBP5L (rat): S811‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cAMP_analog increase
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;

S820-p - STXBP5L (human)
Modsite: YNRsRsSsIssIDKD SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S820‑p, STXBP5L (mouse): S819‑p, STXBP5L iso8 (mouse): S826‑p, STXBP5L (rat): S819‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cAMP_analog increase
glucose increase
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;

S822-p - STXBP5L (human)
Modsite: RsRsSsIssIDKDSK SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S822‑p, STXBP5L (mouse): S821‑p, STXBP5L iso8 (mouse): S828‑p, STXBP5L (rat): S821‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;

S823-p - STXBP5L (human)
Modsite: sRsSsIssIDKDSKE SwissProt Entrez-Gene
Orthologous residues
STXBP5L (human): S823‑p, STXBP5L (mouse): S822‑p, STXBP5L iso8 (mouse): S829‑p, STXBP5L (rat): S822‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Disease tissue studied:  pancreatic cancer, pancreatic carcinoma
Relevant cell lines - cell types - tissues:  293FT, INS-1 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  human, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  protein degradation
Comments:  in a multiple residue S->D mutant;