Curated Information
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Home > Curated Information Page > PubMed Id: 25002506
Galan JA, et al. (2014) Phosphoproteomic analysis identifies the tumor suppressor PDCD4 as a RSK substrate negatively regulated by 14-3-3. Proc Natl Acad Sci U S A 111, E2918-27 25002506
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S280-p - Chk1 (human)
Modsite: AKRPRVtsGGVsEsP SwissProt Entrez-Gene
Orthologous residues
Chk1 (human): S280‑p, Chk1 (mouse): S280‑p, Chk1 (rat): S280‑p, Chk1 (chicken): S280‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), A375 (melanocyte), COLO-829 (melanocyte), Hs852.T (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), A375 (melanocyte), COLO-829 (melanocyte), Hs852.T (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), A375 (melanocyte), COLO-829 (melanocyte), Hs852.T (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human

S67-p - PDCD4 (human)
Modsite: kRRLRKNssRDsGrG SwissProt Entrez-Gene
Orthologous residues
PDCD4 (human): S67‑p, PDCD4 iso2 (human): S56‑p, PDCD4 (mouse): S67‑p, PDCD4 (rat): S67‑p, PDCD4 (cow): S67‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
PD184352 phorbol_ester inhibit treatment-induced increase
BI-D1870 phorbol_ester inhibit treatment-induced increase
SL0101 phorbol_ester inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces pull-down assay
Comments:  PDCD4 degradation

S76-p - PDCD4 (human)
Modsite: RDsGrGDsVsDsGsD SwissProt Entrez-Gene
Orthologous residues
PDCD4 (human): S76‑p, PDCD4 iso2 (human): S65‑p, PDCD4 (mouse): S76‑p, PDCD4 (rat): S76‑p, PDCD4 (cow): S76‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
PD184352 phorbol_ester inhibit treatment-induced increase
BI-D1870 phorbol_ester inhibit treatment-induced increase
SL0101 phorbol_ester inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces pull-down assay
Comments:  PDCD4 degradation

S457-p - PDCD4 (human)
Modsite: RGRKRFVsEGDGGRL SwissProt Entrez-Gene
Orthologous residues
PDCD4 (human): S457‑p, PDCD4 iso2 (human): S446‑p, PDCD4 (mouse): S457‑p, PDCD4 (rat): S457‑p, PDCD4 (cow): S457‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), A375 (melanocyte), COLO-829 (melanocyte), Hs852.T (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE p90RSK (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p90RSK (human) pharmacological inhibitor of upstream enzyme, modification site within consensus motif, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
PD184352 phorbol_ester inhibit treatment-induced increase
BI-D1870 phorbol_ester inhibit treatment-induced increase
SL0101 phorbol_ester inhibit treatment-induced increase
MEK1 (human) increase
Downstream Regulation
Effect of modification (function):  intracellular localization

S235-p - S6 (human)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), A375 (melanocyte), COLO-829 (melanocyte), Hs852.T (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human

S236-p - S6 (human)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), A375 (melanocyte), COLO-829 (melanocyte), Hs852.T (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human

S240-p - S6 (human)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), A375 (melanocyte), COLO-829 (melanocyte), Hs852.T (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human

S244-p - S6 (human)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), A375 (melanocyte), COLO-829 (melanocyte), Hs852.T (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human