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Home > Curated Information Page > PubMed Id: 21195350
Chen S, Wasserman DH, MacKintosh C, Sakamoto K (2011) Mice with AS160/TBC1D4-Thr649Ala knockin mutation are glucose intolerant with reduced insulin sensitivity and altered GLUT4 trafficking. Cell Metab 13, 68-79 21195350
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant
glucose increase
glucose AS160 (human) no effect upon treatment-induced increase T649A mutant
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S324-p - AS160 (mouse)
Modsite: EFRsRCssVTGVMQK SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S318‑p, AS160 iso2 (human): S318‑p, AS160 iso3 (human): S318‑p, AS160 (mouse): S324‑p, AS160 iso2 (mouse): S324‑p, AS160 (rat): S326‑p, AS160 (cow): S319‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant

S348-p - AS160 (mouse)
Modsite: QPRRRHAsAPsHVQP SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S341‑p, AS160 iso2 (human): S341‑p, AS160 iso3 (human): S341‑p, AS160 (mouse): S348‑p, AS160 iso2 (mouse): S348‑p, AS160 (rat): S350‑p, AS160 (cow): S343‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant

S577-p - AS160 (mouse)
Modsite: AKRsLtssLENIFSr SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S570‑p, AS160 iso2 (human): S570‑p, AS160 iso3 (human): S570‑p, AS160 (mouse): S577‑p, AS160 iso2 (mouse): S577‑p, AS160 (rat): S579‑p, AS160 (cow): S572‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant

S595-p - AS160 (mouse)
Modsite: RMRGRLGsMDsFERA SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S588‑p, AS160 iso2 (human): S588‑p, AS160 iso3 (human): S588‑p, AS160 (mouse): S595‑p, AS160 iso2 (mouse): S595‑p, AS160 (rat): S597‑p, AS160 (cow): S590‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant

T649-p - AS160 (mouse)
Modsite: QFRRRAHtFsHPPSS SwissProt Entrez-Gene
Orthologous residues
AS160 (human): T642‑p, AS160 iso2 (human): T642‑p, AS160 iso3 (human): T642‑p, AS160 (mouse): T649‑p, AS160 iso2 (mouse): T649‑p, AS160 (rat): T651‑p, AS160 (cow): T644‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  endocytosis, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 beta (human) Induces co-immunoprecipitation
Comments:  regulates GLUT4 trafficking, induces glucose uptake

S758-p - AS160 (mouse)
Modsite: EGRKRtsstCsNEsL SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S751‑p, AS160 iso2 (human): S688‑p, AS160 iso3 (human): S743‑p, AS160 (mouse): S758‑p, AS160 iso2 (mouse): S695‑p, AS160 (rat): S760‑p, AS160 (cow): S753‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant

S21-p - GSK3A (mouse)
Modsite: sGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant

T590-p - TBC1D1 (mouse)
Modsite: AFRRRANtLsHFPVE SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): T596‑p, TBC1D1 iso2 (human): T596‑p, TBC1D1 (mouse): T590‑p, TBC1D1 iso2 (mouse): T590‑p, TBC1D1 (rat): T590‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', heart
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin AS160 (human) no effect upon treatment-induced increase T649A mutant