Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 22975324
Hopkins S, et al. (2012) Mig6 is a sensor of EGF receptor inactivation that directly activates c-Abl to induce apoptosis during epithelial homeostasis. Dev Cell 23, 547-59 22975324
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

Y412-p - Abl iso2 (human)
Modsite: RLMTGDtyTAHAGAK SwissProt Entrez-Gene
Orthologous residues
Abl (human): Y393‑p, Abl iso2 (human): Y412‑p, Abl (mouse): Y393‑p, Abl iso4 (mouse): Y412‑p, Abl (rat): Y393‑p, Abl iso2 (rat): Y412‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (human) transfection of wild-type enzyme, transfection of constitutively active enzyme, transfection of inactive enzyme, activation of upstream enzyme Autophosphorylation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIG-6 (human) increase
EGF decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  apoptosis, induced

Y488-p - Abl iso2 (human)
Modsite: EGCPEKVyELMRACW SwissProt Entrez-Gene
Orthologous residues
Abl (human): Y469‑p, Abl iso2 (human): Y488‑p, Abl (mouse): Y469‑p, Abl iso4 (mouse): Y488‑p, Abl (rat): Y469‑p, Abl iso2 (rat): Y488‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) genetic knockout/knockin of upstream enzyme, transfection of wild-type enzyme, pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MIG-6 (human) increase
EGF increase
SU6656 EGF inhibit treatment-induced increase
PP2 decrease
Fyn (human), Yes (human) increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Effect of modification (process):  apoptosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
MIG-6 (human) Disrupts co-immunoprecipitation
Comments:  inhibits Mig6-dependent proapoptotic activation, inhibits Abl phosphorylation

Y394-p - MIG-6 (human)
Modsite: KKVsstHyyLLPERP SwissProt Entrez-Gene
Orthologous residues
MIG‑6 (human): Y394‑p, MIG‑6 (mouse): Y393‑p, MIG‑6 (rat): Y391‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of constitutively active enzyme, transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nilotinib decrease

Y395-p - MIG-6 (human)
Modsite: KVsstHyyLLPERPP SwissProt Entrez-Gene
Orthologous residues
MIG‑6 (human): Y395‑p, MIG‑6 (mouse): Y394‑p, MIG‑6 (rat): Y392‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Abl (human) co-immunoprecipitation, transfection of wild-type enzyme, transfection of constitutively active enzyme, transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nilotinib decrease