Xu W, et al. (2012) Dynamic tyrosine phosphorylation modulates cycling of the HSP90-P50(CDC37)-AHA1 chaperone machine. Mol Cell 47, 434-43 22727666

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

Y4-p - CDC37 (human) ▲

Modsite: ____MVDysVWDHIE SwissProt Entrez-Gene Orthologous residues CDC37 (human): Y4‑p, CDC37 (mouse): Y4‑p, CDC37 (rat): Y4‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Yes (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Yes (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PP1 decrease PP2 decrease dasatinib decrease SKI-606 decrease bpV increase imatinib no change compared to control siRNA decrease Yes-siRNA inhibits Downstream Regulation Effect of modification (function):  molecular association, regulation

Y298-p - CDC37 (human) ▲

Modsite: GLDPVEVyEsLPEEL SwissProt Entrez-Gene Orthologous residues CDC37 (human): Y298‑p, CDC37 (mouse): Y299‑p, CDC37 (rat): Y299‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast) Cellular systems studied:  cell lines Species studied:  green monkey, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Yes (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Yes (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PP1 decrease PP2 decrease dasatinib decrease SKI-606 decrease bpV increase imatinib no change compared to control siRNA decrease Yes-siRNA inhibits Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CDK4 (human) Disrupts in vitro, co-immunoprecipitation RAF1 (human) Disrupts in vitro, co-immunoprecipitation

Y197-p - HSP90A (human) ▲

Modsite: LkEDQtEyLEErRIk SwissProt Entrez-Gene Orthologous residues HSP90A (human): Y197‑p, HSP90A iso2 (human): Y319‑p, HSP90A (mouse): Y197‑p, HSP90A (rat): Y197‑p, HSP90A (hamster): Y197‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  mononuclear-blood Cellular systems studied:  cell lines Species studied:  green monkey, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester, ionomycin decrease Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CDK4 (human) Disrupts in vitro CDC37 (human) Disrupts in vitro

Y313-p - HSP90A (human) ▲

Modsite: NEEyGEFyksLtNDW SwissProt Entrez-Gene Orthologous residues HSP90A (human): Y313‑p, HSP90A iso2 (human): Y435‑p, HSP90A (mouse): Y314‑p, HSP90A (rat): Y314‑p, HSP90A (hamster): Y314‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293-A (epithelial) Cellular systems studied:  cell lines Species studied:  green monkey, human Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays AHSA1 (human) Induces

Y627-p - HSP90A (human) ▲

Modsite: RDNstMGyMAAkkHL SwissProt Entrez-Gene Orthologous residues HSP90A (human): Y627‑p, HSP90A iso2 (human): Y749‑p, HSP90A (mouse): Y628‑p, HSP90A (rat): Y628‑p, HSP90A (hamster): Y628‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293-A (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PPP5C (human) Disrupts co-immunoprecipitation