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Home > Curated Information Page > PubMed Id: 22727666
Xu W, et al. (2012) Dynamic tyrosine phosphorylation modulates cycling of the HSP90-P50(CDC37)-AHA1 chaperone machine. Mol Cell 47, 434-43 22727666
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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Y4-p - CDC37 (human)
Modsite: ____MVDysVWDHIE SwissProt Entrez-Gene
Orthologous residues
CDC37 (human): Y4‑p, CDC37 (mouse): Y4‑p, CDC37 (rat): Y4‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Yes (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Yes (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP1 decrease
PP2 decrease
dasatinib decrease
SKI-606 decrease
bpV increase
imatinib no change compared to control
siRNA decrease Yes-siRNA inhibits
Downstream Regulation
Effect of modification (function):  molecular association, regulation

Y298-p - CDC37 (human)
Modsite: GLDPVEVyEsLPEEL SwissProt Entrez-Gene
Orthologous residues
CDC37 (human): Y298‑p, CDC37 (mouse): Y299‑p, CDC37 (rat): Y299‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), COS7 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Yes (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Yes (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PP1 decrease
PP2 decrease
dasatinib decrease
SKI-606 decrease
bpV increase
imatinib no change compared to control
siRNA decrease Yes-siRNA inhibits
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK4 (human) Disrupts in vitro, co-immunoprecipitation
RAF1 (human) Disrupts in vitro, co-immunoprecipitation

Y197-p - HSP90A (human)
Modsite: LkEDQtEyLEErRIk SwissProt Entrez-Gene
Orthologous residues
HSP90A (human): Y197‑p, HSP90A iso2 (human): Y319‑p, HSP90A (mouse): Y197‑p, HSP90A (rat): Y197‑p, HSP90A (hamster): Y197‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  mononuclear-blood
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester, ionomycin decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CDK4 (human) Disrupts in vitro
CDC37 (human) Disrupts in vitro

Y313-p - HSP90A (human)
Modsite: NEEyGEFyksLtNDW SwissProt Entrez-Gene
Orthologous residues
HSP90A (human): Y313‑p, HSP90A iso2 (human): Y435‑p, HSP90A (mouse): Y314‑p, HSP90A (rat): Y314‑p, HSP90A (hamster): Y314‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS7 (fibroblast), HEK293-A (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
AHSA1 (human) Induces

Y627-p - HSP90A (human)
Modsite: RDNstMGyMAAkkHL SwissProt Entrez-Gene
Orthologous residues
HSP90A (human): Y627‑p, HSP90A iso2 (human): Y749‑p, HSP90A (mouse): Y628‑p, HSP90A (rat): Y628‑p, HSP90A (hamster): Y628‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293-A (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP5C (human) Disrupts co-immunoprecipitation