Curated Information
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Home > Curated Information Page > PubMed Id: 24961241
Lee Hamilton D, et al. (2014) Molecular brakes regulating mTORC1 activation in skeletal muscle following synergist ablation. Am J Physiol Endocrinol Metab 307, E365-73 24961241
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S479-p - GRB10 (rat)
Modsite: TRMNILSsQsPLHPS SwissProt Entrez-Gene
Orthologous residues
GRB10 (human): S474‑p, GRB10 iso2 (human): S428‑p, GRB10 (mouse): S501‑p, GRB10 (rat): S479‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
synergist ablation increase

S481-p - GRB10 (rat)
Modsite: MNILSsQsPLHPSTL SwissProt Entrez-Gene
Orthologous residues
GRB10 (human): S476‑p, GRB10 iso2 (human): S430‑p, GRB10 (mouse): S503‑p, GRB10 (rat): S481‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
synergist ablation increase

T389-p - p70S6K iso2 (rat)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
synergist ablation increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, protein degradation
Comments:  degradation of IRS1/2

S939-p - TSC2 (rat)
Modsite: KFRARSTsLNERPKS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S939‑p, TSC2 iso2 (human): S939‑p, TSC2 iso3 (human): S939‑p, TSC2 iso4 (human): S939‑p, TSC2 (mouse): S939‑p, TSC2 iso6 (mouse): S939‑p, TSC2 (rat): S939‑p, TSC2 iso2 (rat): S939‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
synergist ablation increase

S1389-p - TSC2 (rat)
Modsite: QPLsKSSsSPELQTL SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1387‑p, TSC2 iso2 (human): S1344‑p, TSC2 iso3 (human): S1343‑p, TSC2 iso4 (human): S1364‑p, TSC2 (mouse): S1388‑p, TSC2 iso6 (mouse): S1366‑p, TSC2 (rat): S1389‑p, TSC2 iso2 (rat): S1346‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
synergist ablation increase

T1466-p - TSC2 (rat)
Modsite: GLRPRGYtISDSAPS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso2 (human): T1419‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat