Curated Information
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Home > Curated Information Page > PubMed Id: 24962708
Li Y, et al. (2014) LIMK-Dependent Actin Polymerization in Primary Sensory Neurons Promotes the Development of Inflammatory Heat Hyperalgesia in Rats. Sci Signal 7, ra61 24962708
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S978-p - SSH1 (human)
Modsite: sPLKRSHsLAKLGSL SwissProt Entrez-Gene
Orthologous residues
SSH1 (human): S978‑p, SSH1 iso2 (human): , SSH1 iso4 (human): S666‑p, SSH1 (mouse): S970‑p, SSH1 (rat): S961‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Freund's_complete_adjuvant increase

S3-p - Cofilin-1 (rat)
Modsite: _____MAsGVAVsDG SwissProt Entrez-Gene
Orthologous residues
Cofilin‑1 (human): S3‑p, Cofilin‑1 (mouse): S3‑p, Cofilin‑1 (rat): S3‑p, Cofilin‑1 (pig): S3‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, western blotting
Relevant cell lines - cell types - tissues:  'neuron, dorsal root ganglion'-brain
Cellular systems studied:  primary cultured cells
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LIMK1 (human)
KINASE LIMK2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LIMK1 (human) pharmacological activator of upstream enzyme, transfection of dominant-negative enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Freund's_complete_adjuvant increase
Downstream Regulation
Comments:  heat hyperalgesia induced by CFA injection

T508-p - LIMK1 (rat)
Modsite: PDRKKRYtVVGNPYW SwissProt Entrez-Gene
Orthologous residues
LIMK1 (human): T508‑p, LIMK1 (mouse): T508‑p, LIMK1 (rat): T508‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, dorsal root ganglion'-brain
Cellular systems studied:  primary cultured cells
Species studied:  rat

T505-p - LIMK2 (rat)
Modsite: SDRKKRYtVVGNPYW SwissProt Entrez-Gene
Orthologous residues
LIMK2 (human): T505‑p, LIMK2 iso2 (human): T484‑p, LIMK2 iso3 (human): T484‑p, LIMK2 (mouse): T505‑p, LIMK2 (rat): T505‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'neuron, dorsal root ganglion'-brain
Cellular systems studied:  primary cultured cells
Species studied:  rat