Li Y, et al. (2014) LIMK-Dependent Actin Polymerization in Primary Sensory Neurons Promotes the Development of Inflammatory Heat Hyperalgesia in Rats. Sci Signal 7, ra61 24962708

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S978-p - SSH1 (human) ▲
Modsite: sPLKRSHsLAKLGSL SwissProt Entrez-Gene Orthologous residues SSH1 (human): S978‑p, SSH1 iso2 (human): ‑, SSH1 iso4 (human): S666‑p, SSH1 (mouse): S970‑p, SSH1 (rat): S961‑p Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Freund's_complete_adjuvant increase

S3-p - Cofilin-1 (rat) ▲

Modsite: _____MAsGVAVsDG SwissProt Entrez-Gene Orthologous residues Cofilin‑1 (human): S3‑p, Cofilin‑1 (mouse): S3‑p, Cofilin‑1 (rat): S3‑p, Cofilin‑1 (pig): S3‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, western blotting Relevant cell lines - cell types - tissues:  'neuron, dorsal root ganglion'-brain Cellular systems studied:  primary cultured cells Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE LIMK1 (human) KINASE LIMK2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE LIMK1 (human) pharmacological activator of upstream enzyme, transfection of dominant-negative enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Freund's_complete_adjuvant increase Downstream Regulation Comments:  heat hyperalgesia induced by CFA injection

T508-p - LIMK1 (rat) ▲
Modsite: PDRKKRYtVVGNPYW SwissProt Entrez-Gene Orthologous residues LIMK1 (human): T508‑p, LIMK1 (mouse): T508‑p, LIMK1 (rat): T508‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  'neuron, dorsal root ganglion'-brain Cellular systems studied:  primary cultured cells Species studied:  rat

T505-p - LIMK2 (rat) ▲

Modsite: SDRKKRYtVVGNPYW SwissProt Entrez-Gene Orthologous residues LIMK2 (human): T505‑p, LIMK2 iso2 (human): T484‑p, LIMK2 iso3 (human): T484‑p, LIMK2 (mouse): T505‑p, LIMK2 (rat): T505‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  'neuron, dorsal root ganglion'-brain Cellular systems studied:  primary cultured cells Species studied:  rat