Shimizu E, Nakatani T, He Z, Partridge NC (2014) Parathyroid Hormone Regulates Histone Deacetylase (HDAC) 4 through Protein Kinase A-mediated Phosphorylation and Dephosphorylation in Osteoblastic Cells. J Biol Chem 289, 21340-50 24904057

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S246-p - HDAC4 (human) ▲

Modsite: FPLRkTAsEPNLKLR SwissProt Entrez-Gene Orthologous residues HDAC4 (human): S246‑p, HDAC4 (mouse): S245‑p, HDAC4 (rat): S245‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  UMR106 (bone cell) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PTH no change compared to control okadaic_acid increase Downstream Regulation Effect of modification (function):  protein stabilization

S632-p - HDAC4 (human) ▲

Modsite: RPLSRAQssPAsAtF SwissProt Entrez-Gene Orthologous residues HDAC4 (human): S632‑p, HDAC4 (mouse): S629‑p, HDAC4 (rat): S630‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), UMR106 (bone cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PTH increase PGE2 increase okadaic_acid PTH augment treatment-induced increase Downstream Regulation Effect of modification (function):  protein stabilization

S245-p - HDAC4 (rat) ▲

Modsite: FPLRKTAsEPNLKLR SwissProt Entrez-Gene Orthologous residues HDAC4 (human): S246‑p, HDAC4 (mouse): S245‑p, HDAC4 (rat): S245‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  UMR106 (bone cell) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PTH no change compared to control okadaic_acid increase

S466-p - HDAC4 (rat) ▲
Modsite: RPLGRTQsAPLPQNA SwissProt Entrez-Gene Orthologous residues HDAC4 (human): S467‑p, HDAC4 (mouse): S465‑p, HDAC4 (rat): S466‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  UMR106 (bone cell) Cellular systems studied:  cell lines Species studied:  rat

S630-p - HDAC4 (rat) ▲

Modsite: RPLSRAQsSPASATF SwissProt Entrez-Gene Orthologous residues HDAC4 (human): S632‑p, HDAC4 (mouse): S629‑p, HDAC4 (rat): S630‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  UMR106 (bone cell) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PTH increase H-89 PTH inhibit treatment-induced increase okadaic_acid PTH augment treatment-induced increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays AML3 (rat) Disrupts co-immunoprecipitation

T705-p - HDAC4 (rat) ▲
Modsite: CIRGRKAtLEELQTV SwissProt Entrez-Gene Orthologous residues HDAC4 (human): T707‑p, HDAC4 (mouse): T704‑p, HDAC4 (rat): T705‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  bone cancer Relevant cell lines - cell types - tissues:  UMR106 (bone cell) Cellular systems studied:  cell lines Species studied:  rat