Kao SH, et al. (2014) GSK3β controls epithelial-mesenchymal transition and tumor metastasis by CHIP-mediated degradation of Slug. Oncogene 33, 3172-82 23851495

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S9-p - GSK3B (human) ▲

Modsite: SGRPRttsFAEsCkP SwissProt Entrez-Gene Orthologous residues GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p Characterization Methods used to characterize site in vivo:  peptide sequencing Relevant cell lines - cell types - tissues:  lung Cellular systems studied:  tissue Species studied:  human Comments:  NSCLC tumor specimens Downstream Regulation Comments:  In samples with low GSK3b-pSer9 staining most (83.3%) displayed a low expression of Snail2.

S92-p - Snail2 (human) ▲

Modsite: RVsPPPPsDTSsKDH SwissProt Entrez-Gene Orthologous residues Snail2 (human): S92‑p, Snail2 (mouse): S93‑p, Snail2 (rat): S92‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  293 (epithelial), CL1-5 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) transfection of constitutively active enzyme, co-immunoprecipitation, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  protein degradation Effect of modification (process):  cell motility, inhibited, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CHIP (human) Induces protein degradation co-immunoprecipitation Comments:  Snail2 luciferase construct, may affect lung metastasis in NSCLC.

S96-p - Snail2 (human) ▲

Modsite: PPPsDTSsKDHsGSE SwissProt Entrez-Gene Orthologous residues Snail2 (human): S96‑p, Snail2 (mouse): S97‑p, Snail2 (rat): S96‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  293 (epithelial), CL1-5 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) transfection of constitutively active enzyme, co-immunoprecipitation, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  protein degradation Effect of modification (process):  cell motility, inhibited, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CHIP (human) Induces protein degradation co-immunoprecipitation Comments:  Snail2 luciferase construct, may affect lung metastasis in NSCLC.

S100-p - Snail2 (human) ▲

Modsite: DTSsKDHsGSEsPIS SwissProt Entrez-Gene Orthologous residues Snail2 (human): S100‑p, Snail2 (mouse): S101‑p, Snail2 (rat): S100‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  293 (epithelial), CL1-5 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) transfection of constitutively active enzyme, co-immunoprecipitation, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  protein degradation Effect of modification (process):  cell motility, inhibited, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CHIP (human) Induces protein degradation co-immunoprecipitation Comments:  Snail2 luciferase construct, may affect lung metastasis in NSCLC.

S104-p - Snail2 (human) ▲

Modsite: KDHsGSEsPISDEEE SwissProt Entrez-Gene Orthologous residues Snail2 (human): S104‑p, Snail2 (mouse): S105‑p, Snail2 (rat): S104‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  293 (epithelial), CL1-5 (pulmonary) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE GSK3B (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) transfection of constitutively active enzyme, co-immunoprecipitation, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  protein degradation Effect of modification (process):  cell motility, inhibited, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CHIP (human) Induces protein degradation co-immunoprecipitation Comments:  Snail2 luciferase construct, may affect lung metastasis in NSCLC.