Curated Information
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Home > Curated Information Page > PubMed Id: 9335504
Kretzschmar M, Doody J, Massagué J (1997) Opposing BMP and EGF signalling pathways converge on the TGF-beta family mediator Smad1. Nature 389, 618-22 9335504
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S187-p - SMAD1 (human)
Modsite: NSHPFPHsPNSSYPN SwissProt Entrez-Gene
Orthologous residues
SMAD1 (human): S187‑p, SMAD1 (mouse): S187‑p, SMAD1 (rat): S187‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, mink
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  Erk-mediated phosphorylation inhibits the nuclear accumulation of Smad1.

S195-p - SMAD1 (human)
Modsite: PNSSYPNsPGSSSSt SwissProt Entrez-Gene
Orthologous residues
SMAD1 (human): S195‑p, SMAD1 (mouse): S195‑p, SMAD1 (rat): S195‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, mink
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_starvation decrease
HGF increase
EGF increase
wortmannin EGF no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  Erk-mediated phosphorylation inhibits the nuclear accumulation of Smad1.

S206-p - SMAD1 (human)
Modsite: SSStYPHsPTSSDPG SwissProt Entrez-Gene
Orthologous residues
SMAD1 (human): S206‑p, SMAD1 (mouse): S206‑p, SMAD1 (rat): S206‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, mink
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_starvation decrease
HGF increase
EGF increase
wortmannin EGF no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  Erk-mediated phosphorylation inhibits the nuclear accumulation of Smad1.

S214-p - SMAD1 (human)
Modsite: PTSSDPGsPFQMPAD SwissProt Entrez-Gene
Orthologous residues
SMAD1 (human): S214‑p, SMAD1 (mouse): S214‑p, SMAD1 (rat): S214‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast), R-1B/L17 (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, mink
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_starvation decrease
HGF increase
EGF increase
wortmannin EGF no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  Erk-mediated phosphorylation inhibits the nuclear accumulation of Smad1.

S462-p - SMAD1 (human)
Modsite: GsPHNPIssVs____ SwissProt Entrez-Gene
Orthologous residues
SMAD1 (human): S462‑p, SMAD1 (mouse): S462‑p, SMAD1 (rat): S465‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  R-1B/L17 (epithelial)
Cellular systems studied:  cell lines
Species studied:  mink
Enzymes shown to modify site in vitro
Type Enzyme
KINASE BMPR1B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, activation of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_starvation decrease
HGF increase
EGF increase
wortmannin EGF no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  BMP-stimulated phosphorylation of Smad1 carboxy-terminal serines induces nuclear accumulation of Smad1.

S463-p - SMAD1 (human)
Modsite: sPHNPIssVs_____ SwissProt Entrez-Gene
Orthologous residues
SMAD1 (human): S463‑p, SMAD1 (mouse): S463‑p, SMAD1 (rat): S466‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  R-1B/L17 (epithelial)
Cellular systems studied:  cell lines
Species studied:  mink
Enzymes shown to modify site in vitro
Type Enzyme
KINASE BMPR1B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, activation of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_starvation decrease
HGF increase
EGF increase
wortmannin EGF no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  BMP-stimulated phosphorylation of Smad1 carboxy-terminal serines induces nuclear accumulation of Smad1.

S465-p - SMAD1 (human)
Modsite: HNPIssVs_______ SwissProt Entrez-Gene
Orthologous residues
SMAD1 (human): S465‑p, SMAD1 (mouse): S465‑p, SMAD1 (rat): S468‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  R-1B/L17 (epithelial)
Cellular systems studied:  cell lines
Species studied:  mink
Enzymes shown to modify site in vitro
Type Enzyme
KINASE BMPR1B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE BMPR1B (human) transfection of wild-type enzyme, activation of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_starvation decrease
HGF increase
EGF increase
wortmannin EGF no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  BMP-stimulated phosphorylation of Smad1 carboxy-terminal serines induces nuclear accumulation of Smad1.