Davies M, et al. (2014) Novel mechanisms of Na+ retention in obesity: phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK. Am J Physiol Renal Physiol 307, F96-F106 24808538

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T172-p - AMPKA2 (mouse) ▲

Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene Orthologous residues AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  'kidney, cortex' Cellular systems studied:  primary cells, tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet decrease HFD-Decrease in cortex

T58-p - NCCT (mouse) ▲
Modsite: MRtFGYNtIDVVPAY SwissProt Entrez-Gene Orthologous residues NCCT (human): T60‑p, NCCT iso2 (human): T60‑p, NCCT iso3 (human): T60‑p, NCCT (mouse): T58‑p, NCCT (rat): T58‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  'kidney, cortex' Cellular systems studied:  primary cells, tissue Species studied:  mouse

T205-p - NKCC1 (mouse) ▲
Modsite: TNtYYLRtFGHNtMD SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T212‑p, NKCC1 iso3 (human): T212‑p, NKCC1 (mouse): T205‑p, NKCC1 (rat): T203‑p

T210-p - NKCC1 (mouse) ▲
Modsite: LRtFGHNtMDAVPRI SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T217‑p, NKCC1 iso3 (human): T217‑p, NKCC1 (mouse): T210‑p, NKCC1 (rat): T208‑p

T96-p - NKCC2 (mouse) ▲

Modsite: TNtyyLQtFGHNtMD SwissProt Entrez-Gene Orthologous residues NKCC2 (human): T100‑p, NKCC2 (mouse): T96‑p, NKCC2 iso3 (mouse): ‑, NKCC2 (rat): T96‑p, NKCC2 (rabbit): T99‑p Characterization Methods used to characterize site in vivo:  immunoassay, immunoprecipitation, phospho-antibody Relevant cell lines - cell types - tissues:  'kidney, cortex' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase Downstream Regulation Effect of modification (function):  activity, induced

T101-p - NKCC2 (mouse) ▲

Modsite: LQtFGHNtMDAVPKI SwissProt Entrez-Gene Orthologous residues NKCC2 (human): T105‑p, NKCC2 (mouse): T101‑p, NKCC2 iso3 (mouse): T96‑p, NKCC2 (rat): T101‑p, NKCC2 (rabbit): T104‑p Characterization Methods used to characterize site in vivo:  immunoassay, immunoprecipitation, phospho-antibody Relevant cell lines - cell types - tissues:  'kidney, cortex' Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase Downstream Regulation Effect of modification (function):  activity, induced

S126-p - NKCC2 (mouse) ▲

Modsite: GPkVNRPsLLEIHEQ SwissProt Entrez-Gene Orthologous residues NKCC2 (human): S130‑p, NKCC2 (mouse): S126‑p, NKCC2 iso3 (mouse): S121‑p, NKCC2 (rat): S126‑p, NKCC2 (rabbit): S129‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody Relevant cell lines - cell types - tissues:  C57MG (epithelial), kidney Cellular systems studied:  cell lines, tissue Species studied:  mouse Comments:  C57BL/6 mice Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase Downstream Regulation Effect of modification (function):  activity, induced Associated Diseases Diseases Alterations Comments obesity, hyperphagic increased S126 phosphorylation leads to enhanced sodium reabsorption in obesity

S325-p - OSR1 (mouse) ▲

Modsite: VRRVPGssGRLHKtE SwissProt Entrez-Gene Orthologous residues OSR1 (human): S325‑p, OSR1 (mouse): S325‑p, OSR1 (rat): S325‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  'kidney, cortex' Cellular systems studied:  primary cells, tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase high-fat diet increase Downstream Regulation Effect of modification (function):  phosphorylation Comments:  Increased phosphorylation of T96 and T101 of NKCC2

S383-p - STLK3 (mouse) ▲

Modsite: VRRVPGssGHLHKTE SwissProt Entrez-Gene Orthologous residues STLK3 (human): S371‑p, STLK3 (mouse): S383‑p, STLK3 (rat): S380‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  'kidney, cortex' Cellular systems studied:  primary cells, tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes high-fat diet increase high-fat diet increase Downstream Regulation Effect of modification (function):  phosphorylation Comments:  Increased phosphorylation of T96 and T101 of NKCC2