Curated Information
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Home > Curated Information Page > Cst Curation Set #4582
Possemato A (2008) CST Curation Set: 4582; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S573-p - ADCY6 (mouse)
Modsite: LQRtRANsMEGLMPR SwissProt Entrez-Gene
Orthologous residues
ADCY6 (human): S576‑p, ADCY6 (mouse): S573‑p, ADCY6 (rat): S588‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S36-p - BRD2 (mouse)
Modsite: GkRIRKPsLLYEGFE SwissProt Entrez-Gene
Orthologous residues
BRD2 (human): S37‑p, BRD2 iso2 (human): S37‑p, BRD2 (mouse): S36‑p, BRD2 (rat): S36‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S50-p - CCDC75 (mouse)
Modsite: NLRNRQKsVKEEERE SwissProt Entrez-Gene
Orthologous residues
CCDC75 (human): S50‑p, CCDC75 iso2 (human): , CCDC75 (mouse): S50‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S260-p - CCT2 (mouse)
Modsite: GsRVRVDsTAKVAEI SwissProt Entrez-Gene
Orthologous residues
CCT2 (human): S260‑p, CCT2 (mouse): S260‑p, CCT2 (rat): S260‑p, CCT2 (cow): S260‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T154-p - CDR2L (mouse)
Modsite: EKRERRRtIHtFPCL SwissProt Entrez-Gene
Orthologous residues
CDR2L (human): T154‑p, CDR2L (mouse): T154‑p, CDR2L (rat): T154‑p, CDR2L (cow): T154‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S114-p - CEP131 (mouse)
Modsite: SGRRRVAsLsKASsE SwissProt Entrez-Gene
Orthologous residues
CEP131 (human): S114‑p, CEP131 iso2 (human): S114‑p, CEP131 (mouse): S114‑p, CEP131 (rat): S114‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S47-p - DNAJC2 (mouse)
Modsite: KRRNRNAstsFQELE SwissProt Entrez-Gene
Orthologous residues
DNAJC2 (human): S47‑p, DNAJC2 iso2 (human): S47‑p, DNAJC2 (mouse): S47‑p, DNAJC2 (rat): S47‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S527-p - E2A iso2 (mouse)
Modsite: VPRtRtsstDEVLsL SwissProt Entrez-Gene
Orthologous residues
E2A (human): , E2A iso2 (human): S530‑p, E2A (mouse): , E2A iso2 (mouse): S527‑p, E2A (rat): , E2A iso2 (rat): S525‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S406-p - EIF4B (mouse)
Modsite: RPRERHPsWRsEEtQ SwissProt Entrez-Gene
Orthologous residues
EIF4B (human): S406‑p, EIF4B (mouse): S406‑p, EIF4B (rat): S406‑p, EIF4B (cow): S406‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S410-p - FAM13B (mouse)
Modsite: QLDLKNVsDGDKWEE SwissProt Entrez-Gene
Orthologous residues
FAM13B (human): S474‑p, FAM13B (mouse): S410‑p, FAM13B (rat): S411‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S276-p - FBXO6 (mouse)
Modsite: IGRRRRAsDsNtHEG SwissProt Entrez-Gene
Orthologous residues
FBXO6 (human): Q283‑p, FBXO6 (mouse): S276‑p, FBXO6 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S2234-p - FLNC (mouse)
Modsite: LGRERLGsFGsItRQ SwissProt Entrez-Gene
Orthologous residues
FLNC (human): S2233‑p, FLNC iso2 (human): S2200‑p, FLNC (mouse): S2234‑p, FLNC (rat): S2234‑p, FLNC (cow): S2231‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T328-p - FMIP (mouse)
Modsite: TtKRRRPtLGVQLDD SwissProt Entrez-Gene
Orthologous residues
FMIP (human): T328‑p, FMIP (mouse): T328‑p, FMIP (rat): T327‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S667-p - IWS1 (mouse)
Modsite: PQRRRLsstGGQtPR SwissProt Entrez-Gene
Orthologous residues
IWS1 (human): S720‑p, IWS1 iso2 (human): S395‑p, IWS1 iso3 (human): S513‑p, IWS1 (mouse): S667‑p, IWS1 (rat): S666‑p, IWS1 (cow): S723‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1032-p - LARP (mouse)
Modsite: EGRKRCPsQsssRPA SwissProt Entrez-Gene
Orthologous residues
LARP (human): S1056‑p, LARP iso3 (human): S979‑p, LARP (mouse): S1032‑p, LARP (rat): S968‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S31-p - LKB1 (mouse)
Modsite: TFIHRIDstEVIyQP SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): S31‑p, LKB1 iso2 (human): S31‑p, LKB1 (mouse): S31‑p, LKB1 (rat): S31‑p, LKB1 (pig):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S129-p - LOC680959 (mouse)
Modsite: NPNARLRsEENE___ GenPept Entrez-Gene
Orthologous residues
LOC680959 (mouse): S129‑p, LOC680959 (rat): S131‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S507-p - MYPT1 (mouse)
Modsite: TIPRRLAstsDIEEK SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S507‑p, MYPT1 iso2 (human): S507‑p, MYPT1 iso3 (human): S507‑p, MYPT1 iso4 (human): S507‑p, MYPT1 (mouse): S507‑p, MYPT1 (rat): S507‑p, MYPT1 (pig): S507‑p, MYPT1 (chicken): S502‑p, MYPT1 (cow): S507‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S509-p - MYPT1 (mouse)
Modsite: PRRLAstsDIEEKEN SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): S509‑p, MYPT1 iso2 (human): S509‑p, MYPT1 iso3 (human): S509‑p, MYPT1 iso4 (human): S509‑p, MYPT1 (mouse): S509‑p, MYPT1 (rat): S509‑p, MYPT1 (pig): S509‑p, MYPT1 (chicken): S504‑p, MYPT1 (cow): S509‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T21-p - NCBP1 (mouse)
Modsite: QPHkRRKtsDANEtE SwissProt Entrez-Gene
Orthologous residues
NCBP1 (human): T21‑p, NCBP1 (mouse): T21‑p, NCBP1 (rat): T21‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1973-p - NuMA-1 (mouse)
Modsite: RQQRKRVssEtHQGP SwissProt Entrez-Gene
Orthologous residues
NuMA‑1 (human): S1991‑p, NuMA‑1 iso2 (human): S1977‑p, NuMA‑1 (mouse): S1973‑p, NuMA‑1 (rat): S1957‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T49-p - NUP93 (mouse)
Modsite: RLRSRtLtRTsQETA SwissProt Entrez-Gene
Orthologous residues
NUP93 (human): T49‑p, NUP93 iso2 (human): , NUP93 (mouse): T49‑p, NUP93 (rat): T49‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S4275-p - Plectin-1 (mouse)
Modsite: KKRERKtssKSSVRK SwissProt Entrez-Gene
Orthologous residues
Plectin‑1 (human): S4268‑p, Plectin‑1 iso2 (human): S4158‑p, Plectin‑1 iso3 (human): S4154‑p, Plectin‑1 iso4 (human): S4131‑p, Plectin‑1 iso5 (human): S4131‑p, Plectin‑1 iso6 (human): S4135‑p, Plectin‑1 iso8 (human): S4109‑p, Plectin‑1 iso9 (human): S4117‑p, Plectin‑1 (mouse): S4275‑p, Plectin‑1 iso3 (mouse): S4127‑p, Plectin‑1 iso6 (mouse): S4156‑p, Plectin‑1 (rat): S4271‑p, Plectin‑1 iso2 (rat): S4128‑p, Plectin‑1 (cow): S4228‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S373-p - PPIG (mouse)
Modsite: RAQRMRVssGERWIK SwissProt Entrez-Gene
Orthologous residues
PPIG (human): S375‑p, PPIG (mouse): S373‑p, PPIG (rat): S373‑p, PPIG (cow): S375‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S374-p - PPIG (mouse)
Modsite: AQRMRVssGERWIKG SwissProt Entrez-Gene
Orthologous residues
PPIG (human): S376‑p, PPIG (mouse): S374‑p, PPIG (rat): S374‑p, PPIG (cow): S376‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T247-p - PRAS40 (mouse)
Modsite: LPRPRLNtsDFQKLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1215-p - PRRC2C (mouse)
Modsite: PLRQREEsEtRsEss SwissProt Entrez-Gene
Orthologous residues
PRRC2C (human): S1242‑p, PRRC2C iso2 (human): S999‑p, PRRC2C iso3 (human): S1242‑p, PRRC2C iso4 (human): S1242‑p, PRRC2C iso7 (human): S1244‑p, PRRC2C (mouse): S1215‑p, PRRC2C iso5 (mouse): S1215‑p, PRRC2C (rat): S1231‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T411-p - QSK (mouse)
Modsite: YLSMRRHtVGVADPR SwissProt Entrez-Gene
Orthologous residues
QSK (human): T469‑p, QSK iso6 (human): , QSK iso7 (human): T469‑p, QSK iso8 (human): T421‑p, QSK (mouse): T411‑p, QSK (rat): T469‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T1133-p - RICTOR (mouse)
Modsite: TSNRRIRtLtEPsVD SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1133‑p, RICTOR iso3 (human): T1133‑p, RICTOR iso4 (human): , RICTOR (mouse): T1133‑p, RICTOR (rat): T1118‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S131-p - RPL32 (mouse)
Modsite: NPNARLRsEENE___ SwissProt Entrez-Gene
Orthologous residues
RPL32 (human): S131‑p, RPL32 (mouse): S131‑p, RPL32 (dog): S131‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S235-p - S6 (mouse)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S236-p - S6 (mouse)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S240-p - S6 (mouse)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S244-p - S6 (mouse)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S816-p - SEMA4B (mouse)
Modsite: RPRVRLGsEIRDSVV SwissProt Entrez-Gene
Orthologous residues
SEMA4B (human): S830‑p, SEMA4B iso3 (human): S830‑p, SEMA4B (mouse): S816‑p, SEMA4B (rat): S814‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S307-p - SGTA (mouse)
Modsite: VVRsRtPsAsHEEQQ SwissProt Entrez-Gene
Orthologous residues
SGTA (human): S305‑p, SGTA (mouse): S307‑p, SGTA (rat): S306‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S607-p - SSH1 (mouse)
Modsite: TGRWRRAstQLDRsL SwissProt Entrez-Gene
Orthologous residues
SSH1 (human): P605‑p, SSH1 iso2 (human): P605‑p, SSH1 iso4 (human): P293‑p, SSH1 (mouse): S607‑p, SSH1 (rat): S569‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S132-p - TRAPPC1 (mouse)
Modsite: LFRSRLDsYVRSLPF SwissProt Entrez-Gene
Orthologous residues
TRAPPC1 (human): S132‑p, TRAPPC1 (mouse): S132‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1026-p - TRIM24 (mouse)
Modsite: EAEDCKFsDDsDDDF SwissProt Entrez-Gene
Orthologous residues
TRIM24 (human): S1025‑p, TRIM24 iso2 (human): S991‑p, TRIM24 (mouse): S1026‑p, TRIM24 (rat): S582‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1029-p - TRIM24 (mouse)
Modsite: DCKFsDDsDDDFVQP SwissProt Entrez-Gene
Orthologous residues
TRIM24 (human): S1028‑p, TRIM24 iso2 (human): S994‑p, TRIM24 (mouse): S1029‑p, TRIM24 (rat): S585‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1043-p - TRIM24 (mouse)
Modsite: PRKKRLKstEDRQLL SwissProt Entrez-Gene
Orthologous residues
TRIM24 (human): S1042‑p, TRIM24 iso2 (human): S1008‑p, TRIM24 (mouse): S1043‑p, TRIM24 (rat): S599‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S3395-p - USP34 (mouse)
Modsite: PVKRRRVssDEEHtV SwissProt Entrez-Gene
Orthologous residues
USP34 (human): S3358‑p, USP34 iso2 (human): S3207‑p, USP34 (mouse): S3395‑p, USP34 (rat): S3399‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S3396-p - USP34 (mouse)
Modsite: VKRRRVssDEEHtVD SwissProt Entrez-Gene
Orthologous residues
USP34 (human): S3359‑p, USP34 iso2 (human): S3208‑p, USP34 (mouse): S3396‑p, USP34 (rat): S3400‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S119-p - YT521 (mouse)
Modsite: DRKIRLsssSsREPY SwissProt Entrez-Gene
Orthologous residues
YT521 (human): S119‑p, YT521 iso2 (human): S119‑p, YT521 (mouse): S119‑p, YT521 (rat): S119‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S120-p - YT521 (mouse)
Modsite: RKIRLsssSsREPYK SwissProt Entrez-Gene
Orthologous residues
YT521 (human): S120‑p, YT521 iso2 (human): S120‑p, YT521 (mouse): S120‑p, YT521 (rat): S120‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse