Curated Information
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Home > Curated Information Page > Cst Curation Set #4579
Possemato A (2008) CST Curation Set: 4579; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S414-p - ACTR10 (mouse)
Modsite: PLMkRAFstEK____ SwissProt Entrez-Gene
Orthologous residues
ACTR10 (human): S414‑p, ACTR10 (mouse): S414‑p, ACTR10 (rat): S414‑p, ACTR10 (cow): S414‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S573-p - ADCY6 (mouse)
Modsite: LQRtRANsMEGLMPR SwissProt Entrez-Gene
Orthologous residues
ADCY6 (human): S576‑p, ADCY6 (mouse): S573‑p, ADCY6 (rat): S588‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S5555-p - AHNAK (mouse)
Modsite: KPRHRsNsFsDEREF SwissProt Entrez-Gene
Orthologous residues
AHNAK (human): S5782‑p, AHNAK iso2 (human): , AHNAK (mouse): S5555‑p, AHNAK (rat): S5349‑p, AHNAK iso2 (rat): S4962‑p, AHNAK (cow): S5404‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S613-p - AKAP12 (mouse)
Modsite: KKRVRRPsEsDKEEE SwissProt Entrez-Gene
Orthologous residues
AKAP12 (human): S627‑p, AKAP12 iso2 (human): S529‑p, AKAP12 iso3 (human): S522‑p, AKAP12 (mouse): S613‑p, AKAP12 (rat): S614‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S123-p - APTX (mouse)
Modsite: TQRKRKRsDCDsEEM SwissProt Entrez-Gene
Orthologous residues
APTX (human): S132‑p, APTX (mouse): S123‑p, APTX (rat): P116‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S36-p - BRD2 (mouse)
Modsite: GkRIRKPsLLYEGFE SwissProt Entrez-Gene
Orthologous residues
BRD2 (human): S37‑p, BRD2 iso2 (human): S37‑p, BRD2 (mouse): S36‑p, BRD2 (rat): S36‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S527-p - E2A iso2 (mouse)
Modsite: VPRtRtsstDEVLsL SwissProt Entrez-Gene
Orthologous residues
E2A (human): , E2A iso2 (human): S530‑p, E2A (mouse): , E2A iso2 (mouse): S527‑p, E2A (rat): , E2A iso2 (rat): S525‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T574-p - eIF3-theta (mouse)
Modsite: RILARRQtIEERkER SwissProt Entrez-Gene
Orthologous residues
eIF3‑theta (human): T574‑p, eIF3‑theta (mouse): T574‑p, eIF3‑theta (rat): T574‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S406-p - EIF4B (mouse)
Modsite: RPRERHPsWRsEEtQ SwissProt Entrez-Gene
Orthologous residues
EIF4B (human): S406‑p, EIF4B (mouse): S406‑p, EIF4B (rat): S406‑p, EIF4B (cow): S406‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S276-p - FBXO6 (mouse)
Modsite: IGRRRRAsDsNtHEG SwissProt Entrez-Gene
Orthologous residues
FBXO6 (human): Q283‑p, FBXO6 (mouse): S276‑p, FBXO6 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T328-p - FMIP (mouse)
Modsite: TtKRRRPtLGVQLDD SwissProt Entrez-Gene
Orthologous residues
FMIP (human): T328‑p, FMIP (mouse): T328‑p, FMIP (rat): T327‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S667-p - IWS1 (mouse)
Modsite: PQRRRLsstGGQtPR SwissProt Entrez-Gene
Orthologous residues
IWS1 (human): S720‑p, IWS1 iso2 (human): S395‑p, IWS1 iso3 (human): S513‑p, IWS1 (mouse): S667‑p, IWS1 (rat): S666‑p, IWS1 (cow): S723‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S458-p - lamin A/C (mouse)
Modsite: FVRLRNKsNEDQsMG SwissProt Entrez-Gene
Orthologous residues
lamin A/C (human): S458‑p, lamin A/C iso2 (human): S458‑p, lamin A/C iso3 (human): S458‑p, lamin A/C (mouse): S458‑p, lamin A/C iso3 (mouse): S346‑p, lamin A/C (rat): S458‑p, lamin A/C (cow): S458‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S31-p - LKB1 (mouse)
Modsite: TFIHRIDstEVIyQP SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): S31‑p, LKB1 iso2 (human): S31‑p, LKB1 (mouse): S31‑p, LKB1 (rat): S31‑p, LKB1 (pig):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S129-p - LOC680959 (mouse)
Modsite: NPNARLRsEENE___ GenPept Entrez-Gene
Orthologous residues
LOC680959 (mouse): S129‑p, LOC680959 (rat): S131‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T540-p - MPRIP (mouse)
Modsite: RREGRsKtFDWAEFR SwissProt Entrez-Gene
Orthologous residues
MPRIP (human): T542‑p, MPRIP iso2 (human): T542‑p, MPRIP iso3 (human): T504‑p, MPRIP (mouse): T540‑p, MPRIP iso6 (mouse): T504‑p, MPRIP (rat): T545‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1973-p - NuMA-1 (mouse)
Modsite: RQQRKRVssEtHQGP SwissProt Entrez-Gene
Orthologous residues
NuMA‑1 (human): S1991‑p, NuMA‑1 iso2 (human): S1977‑p, NuMA‑1 (mouse): S1973‑p, NuMA‑1 (rat): S1957‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S104-p - PEA-15 (mouse)
Modsite: TkLtRIPsAKKyKDI SwissProt Entrez-Gene
Orthologous residues
PEA‑15 (human): S104‑p, PEA‑15 iso2 (human): S125‑p, PEA‑15 (mouse): S104‑p, PEA‑15 (rat): S104‑p, PEA‑15 (hamster): S104‑p, PEA‑15 (cow): S104‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S116-p - PEA-15 (mouse)
Modsite: KDIIRQPsEEEIIKL SwissProt Entrez-Gene
Orthologous residues
PEA‑15 (human): S116‑p, PEA‑15 iso2 (human): S137‑p, PEA‑15 (mouse): S116‑p, PEA‑15 (rat): S116‑p, PEA‑15 (hamster): S116‑p, PEA‑15 (cow): S116‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T4274-p - Plectin-1 (mouse)
Modsite: EKKRERKtssKSSVR SwissProt Entrez-Gene
Orthologous residues
Plectin‑1 (human): T4267‑p, Plectin‑1 iso2 (human): T4157‑p, Plectin‑1 iso3 (human): T4153‑p, Plectin‑1 iso4 (human): T4130‑p, Plectin‑1 iso5 (human): T4130‑p, Plectin‑1 iso6 (human): T4134‑p, Plectin‑1 iso8 (human): T4108‑p, Plectin‑1 iso9 (human): T4116‑p, Plectin‑1 (mouse): T4274‑p, Plectin‑1 iso3 (mouse): T4126‑p, Plectin‑1 iso6 (mouse): T4155‑p, Plectin‑1 (rat): T4270‑p, Plectin‑1 iso2 (rat): T4127‑p, Plectin‑1 (cow): T4227‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S4275-p - Plectin-1 (mouse)
Modsite: KKRERKtssKSSVRK SwissProt Entrez-Gene
Orthologous residues
Plectin‑1 (human): S4268‑p, Plectin‑1 iso2 (human): S4158‑p, Plectin‑1 iso3 (human): S4154‑p, Plectin‑1 iso4 (human): S4131‑p, Plectin‑1 iso5 (human): S4131‑p, Plectin‑1 iso6 (human): S4135‑p, Plectin‑1 iso8 (human): S4109‑p, Plectin‑1 iso9 (human): S4117‑p, Plectin‑1 (mouse): S4275‑p, Plectin‑1 iso3 (mouse): S4127‑p, Plectin‑1 iso6 (mouse): S4156‑p, Plectin‑1 (rat): S4271‑p, Plectin‑1 iso2 (rat): S4128‑p, Plectin‑1 (cow): S4228‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S374-p - PPIG (mouse)
Modsite: AQRMRVssGERWIKG SwissProt Entrez-Gene
Orthologous residues
PPIG (human): S376‑p, PPIG (mouse): S374‑p, PPIG (rat): S374‑p, PPIG (cow): S376‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T247-p - PRAS40 (mouse)
Modsite: LPRPRLNtsDFQKLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1215-p - PRRC2C (mouse)
Modsite: PLRQREEsEtRsEss SwissProt Entrez-Gene
Orthologous residues
PRRC2C (human): S1242‑p, PRRC2C iso2 (human): S999‑p, PRRC2C iso3 (human): S1242‑p, PRRC2C iso4 (human): S1242‑p, PRRC2C iso7 (human): S1244‑p, PRRC2C (mouse): S1215‑p, PRRC2C iso5 (mouse): S1215‑p, PRRC2C (rat): S1231‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T411-p - QSK (mouse)
Modsite: YLSMRRHtVGVADPR SwissProt Entrez-Gene
Orthologous residues
QSK (human): T469‑p, QSK iso6 (human): , QSK iso7 (human): T469‑p, QSK iso8 (human): T421‑p, QSK (mouse): T411‑p, QSK (rat): T469‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S29-p - RALBP1 (mouse)
Modsite: sGLtRtPssEEIsPT SwissProt Entrez-Gene
Orthologous residues
RALBP1 (human): S29‑p, RALBP1 (mouse): S29‑p, RALBP1 (rat): S29‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T753-p - RALGAPA1 (mouse)
Modsite: RSIVRQKtVDIDDAQ SwissProt Entrez-Gene
Orthologous residues
RALGAPA1 (human): T754‑p, RALGAPA1 iso2 (human): T754‑p, RALGAPA1 iso3 (human): T754‑p, RALGAPA1 (mouse): T753‑p, RALGAPA1 iso7 (mouse): T753‑p, RALGAPA1 iso8 (mouse): T753‑p, RALGAPA1 (rat): T753‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T766-p - RALGAPA1 (mouse)
Modsite: AQILPRStRVRHFsQ SwissProt Entrez-Gene
Orthologous residues
RALGAPA1 (human): T767‑p, RALGAPA1 iso2 (human): T767‑p, RALGAPA1 iso3 (human): T814‑p, RALGAPA1 (mouse): T766‑p, RALGAPA1 iso7 (mouse): T766‑p, RALGAPA1 iso8 (mouse): T813‑p, RALGAPA1 (rat): T766‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S89-p - RDBP (mouse)
Modsite: KNSGFKRsRtLEGKL SwissProt Entrez-Gene
Orthologous residues
RDBP (human): S89‑p, RDBP iso3 (human): S96‑p, RDBP (mouse): S89‑p, RDBP (rat): S89‑p, RDBP (cow): S89‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T1133-p - RICTOR (mouse)
Modsite: TSNRRIRtLtEPsVD SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1133‑p, RICTOR iso3 (human): T1133‑p, RICTOR iso4 (human): , RICTOR (mouse): T1133‑p, RICTOR (rat): T1118‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S131-p - RPL32 (mouse)
Modsite: NPNARLRsEENE___ SwissProt Entrez-Gene
Orthologous residues
RPL32 (human): S131‑p, RPL32 (mouse): S131‑p, RPL32 (dog): S131‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S235-p - S6 (mouse)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S236-p - S6 (mouse)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S240-p - S6 (mouse)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1490-p - Scribble (mouse)
Modsite: WRAARMKsLEQDALR SwissProt Entrez-Gene
Orthologous residues
Scribble (human): S1508‑p, Scribble iso2 (human): S1427‑p, Scribble iso3 (human): S1508‑p, Scribble (mouse): S1490‑p, Scribble iso3 (mouse): S1518‑p, Scribble (rat): S1516‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T305-p - SGTA (mouse)
Modsite: sQVVRsRtPsAsHEE SwissProt Entrez-Gene
Orthologous residues
SGTA (human): T303‑p, SGTA (mouse): T305‑p, SGTA (rat): T304‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S182-p - STX18 (mouse)
Modsite: TKRKDsTsEKAPQNA SwissProt Entrez-Gene
Orthologous residues
STX18 (human): S182‑p, STX18 (mouse): S182‑p, STX18 iso2 (mouse): S156‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S132-p - TRAPPC1 (mouse)
Modsite: LFRSRLDsYVRSLPF SwissProt Entrez-Gene
Orthologous residues
TRAPPC1 (human): S132‑p, TRAPPC1 (mouse): S132‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S3396-p - USP34 (mouse)
Modsite: VKRRRVssDEEHtVD SwissProt Entrez-Gene
Orthologous residues
USP34 (human): S3359‑p, USP34 iso2 (human): S3208‑p, USP34 (mouse): S3396‑p, USP34 (rat): S3400‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse