Curated Information
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Home > Curated Information Page > Cst Curation Set #4895
Possemato A (2008) CST Curation Set: 4895; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S635-p - AAK1 (mouse)
Modsite: AGHRRILsDVtHsAV SwissProt Entrez-Gene
Orthologous residues
AAK1 (human): S637‑p, AAK1 iso2 (human): S637‑p, AAK1 (mouse): S635‑p, AAK1 (rat): S638‑p, AAK1 (cow): S633‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S35-p - AHNAK2 iso2 (mouse)
Modsite: KGRSRKLsFsMPRLA SwissProt Entrez-Gene
Orthologous residues
AHNAK2 (human): G5020‑p, AHNAK2 iso2 (human): G18‑p, AHNAK2 iso3 (human): G4920‑p, AHNAK2 (mouse): , AHNAK2 iso2 (mouse): S35‑p, AHNAK2 (rat): S195‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S348-p - AS160 (mouse)
Modsite: QPRRRHAsAPsHVQP SwissProt Entrez-Gene
Orthologous residues
AS160 (human): S341‑p, AS160 iso2 (human): S341‑p, AS160 iso3 (human): S341‑p, AS160 (mouse): S348‑p, AS160 iso2 (mouse): S348‑p, AS160 (rat): S350‑p, AS160 (cow): S343‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S63-p - ATF-1 (mouse)
Modsite: GILARRPsYRKILKD SwissProt Entrez-Gene
Orthologous residues
ATF‑1 (human): S63‑p, ATF‑1 (mouse): S63‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S1119-p - ATRX (mouse)
Modsite: DLRERRNssSKRNTK SwissProt Entrez-Gene
Orthologous residues
ATRX (human): L1140‑p, ATRX iso2 (human): L936‑p, ATRX iso4 (human): L1102‑p, ATRX iso6 (human): L1072‑p, ATRX (mouse): S1119‑p, ATRX (rat): S1113‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S506-p - C2CD2 (mouse)
Modsite: LKsPRKKstIIISGI SwissProt Entrez-Gene
Orthologous residues
C2CD2 (human): S511‑p, C2CD2 (mouse): S506‑p, C2CD2 (rat): S506‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S429-p - CLCC1 (mouse)
Modsite: ALRQRFHsGNKsPEV SwissProt Entrez-Gene
Orthologous residues
CLCC1 (human): T434‑p, CLCC1 (mouse): S429‑p, CLCC1 (rat): S434‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S896-p - COBLL1 (mouse)
Modsite: RHLHRtLssPtGtEt SwissProt Entrez-Gene
Orthologous residues
COBLL1 (human): S813‑p, COBLL1 iso2 (human): S851‑p, COBLL1 (mouse): S896‑p, COBLL1 iso2 (mouse): S864‑p, COBLL1 (rat): S835‑p, COBLL1 (cow):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S161-p - EDC3 (mouse)
Modsite: SFRRRHNsWsSssRH SwissProt Entrez-Gene
Orthologous residues
EDC3 (human): S161‑p, EDC3 (mouse): S161‑p, EDC3 (rat): S160‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S2152-p - FLNA (mouse)
Modsite: TRRRRAPsVANIGsH SwissProt Entrez-Gene
Orthologous residues
FLNA (human): S2152‑p, FLNA iso2 (human): S2144‑p, FLNA (mouse): S2152‑p, FLNA (rat): S2144‑p, FLNA (cow): S2155‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S241-p - MDH1 (mouse)
Modsite: VIKARKLssAMsAAK SwissProt Entrez-Gene
Orthologous residues
MDH1 (human): S241‑p, MDH1 iso2 (human): S152‑p, MDH1 iso3  (human): S259‑p, MDH1 (mouse): S241‑p, MDH1 (rat): S241‑p, MDH1 (pig): S241‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S2152-p - Notch 1 (mouse)
Modsite: GkkARkPsTkGLACG SwissProt Entrez-Gene
Orthologous residues
Notch 1 (human): S2162‑p, Notch 1 (mouse): S2152‑p, Notch 1 (rat): S2152‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T223-p - perilipin 1 (mouse)
Modsite: SLVRRVstLANtLSR SwissProt Entrez-Gene
Orthologous residues
perilipin 1 (human): A224‑p, perilipin 1 (mouse): T223‑p, perilipin 1 iso3 (mouse): T223‑p, perilipin 1 (rat): T224‑p, perilipin 1 iso2 (rat): T224‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T49-p - PIK3C2A (mouse)
Modsite: LQKDRQMtDsPRGFE SwissProt Entrez-Gene
Orthologous residues
PIK3C2A (human): T49‑p, PIK3C2A (mouse): T49‑p, PIK3C2A (rat): T49‑p, PIK3C2A (cow): T49‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S51-p - PIK3C2A (mouse)
Modsite: KDRQMtDsPRGFELS SwissProt Entrez-Gene
Orthologous residues
PIK3C2A (human): N51‑p, PIK3C2A (mouse): S51‑p, PIK3C2A (rat): S51‑p, PIK3C2A (cow): N51‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S235-p - S6 (mouse)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S236-p - S6 (mouse)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S240-p - S6 (mouse)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S514-p - Tks5 (mouse)
Modsite: PNLSRRtstLTRPKV SwissProt Entrez-Gene
Orthologous residues
Tks5 (human): S515‑p, Tks5 iso2 (human): S322‑p, Tks5 (mouse): S514‑p, Tks5 (rat): S463‑p, Tks5 (cow): S376‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S454-p - TRIM3 (mouse)
Modsite: QKAVRRPssMystGG SwissProt Entrez-Gene
Orthologous residues
TRIM3 (human): S454‑p, TRIM3 (mouse): S454‑p, TRIM3 (rat): S454‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

S645-p - vigilin (mouse)
Modsite: AARSRILsIQKDLAN SwissProt Entrez-Gene
Orthologous residues
vigilin (human): S645‑p, vigilin iso2 (human): S612‑p, vigilin (mouse): S645‑p, vigilin (rat): S645‑p, vigilin (cow): S645‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse