Curated Information
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Home > Curated Information Page > Cst Curation Set #2771
Guo A (2007) CST Curation Set: 2771; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S873-p - AKAP2 (mouse)
Modsite: TRVNRRKsALALRWE SwissProt Entrez-Gene
Orthologous residues
AKAP2 (human): S839‑p, AKAP2 iso4 (human): S1083‑p, AKAP2 iso6 (human): S1070‑p, AKAP2 (mouse): S873‑p, AKAP2 iso2 (mouse): S860‑p, AKAP2 iso3 (mouse): , AKAP2 iso6 (mouse): S901‑p, AKAP2 (rat): S850‑p, AKAP2 (cow): S835‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S1184-p - capicua (mouse)
Modsite: CNKDRKKsSsEAKPA SwissProt Entrez-Gene
Orthologous residues
capicua (human): S277‑p, capicua (mouse): S1184‑p, capicua iso2 (mouse): S277‑p, capicua (rat): S277‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S520-p - CDK17 (mouse)
Modsite: HGKNRRQsMLF____ SwissProt Entrez-Gene
Orthologous residues
CDK17 (human): S520‑p, CDK17 iso2 (human): , CDK17 (mouse): S520‑p, CDK17 (rat): , CDK17 (cow): S523‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T238-p - CROP (mouse)
Modsite: KEKLRKRtEEPDRDE SwissProt Entrez-Gene
Orthologous residues
CROP (human): T238‑p, CROP (mouse): T238‑p, CROP (rat): T238‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S105-p - DR1 (mouse)
Modsite: ALKRRKAssRLENLG SwissProt Entrez-Gene
Orthologous residues
DR1 (human): S105‑p, DR1 (mouse): S105‑p, DR1 (rat): S105‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T574-p - eIF3-theta (mouse)
Modsite: RILARRQtIEERkER SwissProt Entrez-Gene
Orthologous residues
eIF3‑theta (human): T574‑p, eIF3‑theta (mouse): T574‑p, eIF3‑theta (rat): T574‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T2180-p - FLNC (mouse)
Modsite: ERLTRTFtRSSHTYT SwissProt Entrez-Gene
Orthologous residues
FLNC (human): T2179‑p, FLNC iso2 (human): T2146‑p, FLNC (mouse): T2180‑p, FLNC (rat): T2180‑p, FLNC (cow): T2177‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S2234-p - FLNC (mouse)
Modsite: LGRERLGsFGsItRQ SwissProt Entrez-Gene
Orthologous residues
FLNC (human): S2233‑p, FLNC iso2 (human): S2200‑p, FLNC (mouse): S2234‑p, FLNC (rat): S2234‑p, FLNC (cow): S2231‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S784-p - GTF2I (mouse)
Modsite: GVPFRRPstFGIPRL SwissProt Entrez-Gene
Orthologous residues
GTF2I (human): S784‑p, GTF2I iso2 (human): S743‑p, GTF2I iso4 (human): S763‑p, GTF2I (mouse): S784‑p, GTF2I iso8 (mouse): S744‑p, GTF2I (rat): S765‑p, GTF2I iso2 (rat): S699‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T265-p - HSC70 (mouse)
Modsite: RAVRRLRtACERAKR SwissProt Entrez-Gene
Orthologous residues
HSC70 (human): T265‑p, HSC70 iso2 (human): T265‑p, HSC70 (mouse): T265‑p, HSC70 (rat): T265‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T265-p - HSP70 (mouse)
Modsite: RAVRRLRtACERAKR SwissProt Entrez-Gene
Orthologous residues
HSP70 (human): T265‑p, HSP70 iso2 (human): T210‑p, HSP70 (mouse): T265‑p, HSP70 (rat): T265‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T267-p - HSPA1L (mouse)
Modsite: RAVRRLRtACERAKR SwissProt Entrez-Gene
Orthologous residues
HSPA1L (human): T267‑p, HSPA1L (mouse): T267‑p, HSPA1L (rat): T267‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T268-p - HSPA2 (mouse)
Modsite: RAVRRLRtACERAKR SwissProt Entrez-Gene
Orthologous residues
HSPA2 (human): T268‑p, HSPA2 (mouse): T268‑p, HSPA2 (rat): T268‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T160-p - KIAA1522 (mouse)
Modsite: RRRERRstVLGLPQH SwissProt Entrez-Gene
Orthologous residues
KIAA1522 (human): T162‑p, KIAA1522 iso2 (human): T221‑p, KIAA1522 (mouse): T160‑p, KIAA1522 iso2 (mouse): T217‑p, KIAA1522 (rat): T160‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T10-p - lamin A/C (mouse)
Modsite: tPsQRRAtRsGAQAs SwissProt Entrez-Gene
Orthologous residues
lamin A/C (human): T10‑p, lamin A/C iso2 (human): T10‑p, lamin A/C iso3 (human): T10‑p, lamin A/C (mouse): T10‑p, lamin A/C iso3 (mouse): , lamin A/C (rat): T10‑p, lamin A/C (cow): T10‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S188-p - matrin 3 (mouse)
Modsite: KRHFRRDsFDDRGPs SwissProt Entrez-Gene
Orthologous residues
matrin 3 (human): S188‑p, matrin 3 iso2 (human): , matrin 3 iso3 (human): S188‑p, matrin 3 (mouse): S188‑p, matrin 3 (rat): S188‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T540-p - MPRIP (mouse)
Modsite: RREGRsKtFDWAEFR SwissProt Entrez-Gene
Orthologous residues
MPRIP (human): T542‑p, MPRIP iso2 (human): T542‑p, MPRIP iso3 (human): T504‑p, MPRIP (mouse): T540‑p, MPRIP iso6 (mouse): T504‑p, MPRIP (rat): T545‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T418-p - nAChRD (mouse)
Modsite: HGLARRLtTARRPPA SwissProt Entrez-Gene
Orthologous residues
nAChRD (human): T415‑p, nAChRD (mouse): T418‑p, nAChRD (rat): T415‑p, nAChRD (torpedo): T418‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T21-p - NCBP1 (mouse)
Modsite: QPHkRRKtsDANEtE SwissProt Entrez-Gene
Orthologous residues
NCBP1 (human): T21‑p, NCBP1 (mouse): T21‑p, NCBP1 (rat): T21‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T616-p - PHACTR4 (mouse)
Modsite: REIKRRLtRKLsQRP SwissProt Entrez-Gene
Orthologous residues
PHACTR4 (human): T624‑p, PHACTR4 iso2 (human): T634‑p, PHACTR4 (mouse): T616‑p, PHACTR4 (rat): T632‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S620-p - PHACTR4 (mouse)
Modsite: RRLtRKLsQRPtVAE SwissProt Entrez-Gene
Orthologous residues
PHACTR4 (human): S628‑p, PHACTR4 iso2 (human): S638‑p, PHACTR4 (mouse): S620‑p, PHACTR4 (rat): S636‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T778-p - PKN1 (mouse)
Modsite: GyGDRtstFCGtPEF SwissProt Entrez-Gene
Orthologous residues
PKN1 (human): T774‑p, PKN1 iso2 (human): T780‑p, PKN1 (mouse): T778‑p, PKN1 (rat): T778‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T815-p - PKN2 (mouse)
Modsite: GyGDRtstFCGtPEF SwissProt Entrez-Gene
Orthologous residues
PKN2 (human): T816‑p, PKN2 iso2 (human): T800‑p, PKN2 (mouse): T815‑p, PKN2 (rat): T817‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T247-p - PRAS40 (mouse)
Modsite: LPRPRLNtsDFQKLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T411-p - QSK (mouse)
Modsite: YLSMRRHtVGVADPR SwissProt Entrez-Gene
Orthologous residues
QSK (human): T469‑p, QSK iso6 (human): , QSK iso7 (human): T469‑p, QSK iso8 (human): T421‑p, QSK (mouse): T411‑p, QSK (rat): T469‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T1080-p - RAPGEF1 (mouse)
Modsite: RNItRRKtDREEKT_ SwissProt Entrez-Gene
Orthologous residues
RAPGEF1 (human): T1071‑p, RAPGEF1 iso2 (human): T1032‑p, RAPGEF1 iso3 (human): T1089‑p, RAPGEF1 (mouse): T1080‑p, RAPGEF1 iso2 (mouse): T1212‑p, RAPGEF1 (rat): T1007‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S235-p - S6 (mouse)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S236-p - S6 (mouse)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S240-p - S6 (mouse)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S244-p - S6 (mouse)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S73-p - SNX12 (mouse)
Modsite: SCVRRRysDFEWLKN SwissProt Entrez-Gene
Orthologous residues
SNX12 (human): S73‑p, SNX12 iso1 (human): S73‑p, SNX12 iso3 (human): S69‑p, SNX12 (mouse): S73‑p, SNX12 (rat): S73‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S72-p - SNX3 (mouse)
Modsite: STVRRrysDFEWLRS SwissProt Entrez-Gene
Orthologous residues
SNX3 (human): S72‑p, SNX3 iso2 (human): , SNX3 iso3 (human): S72‑p, SNX3 iso4 (human): S50‑p, SNX3 (mouse): S72‑p, SNX3 (rat): S72‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S224-p - SRGAP2 (mouse)
Modsite: EKHVRRSsVKKIEKM SwissProt Entrez-Gene
Orthologous residues
SRGAP2 (human): S224‑p, SRGAP2 iso2 (human): S224‑p, SRGAP2 (mouse): S224‑p, SRGAP2 (rat): S224‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T616-p - supervillin (mouse)
Modsite: KTSERFRtQPITSAE SwissProt Entrez-Gene
Orthologous residues
supervillin (human): T657‑p, supervillin iso2 (human): , supervillin iso3 (human): T630‑p, supervillin iso4 (human): T657‑p, supervillin (mouse): T616‑p, supervillin iso4 (mouse): , supervillin (rat): T611‑p, supervillin (ferret):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T629-p - SYNPO2L (mouse)
Modsite: QEARRRGtRKQMFRP SwissProt Entrez-Gene
Orthologous residues
SYNPO2L (human): T632‑p, SYNPO2L (mouse): T629‑p, SYNPO2L (rat): T632‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

T487-p - UBXN4 (mouse)
Modsite: GKIyRLRtQDDGEDE SwissProt Entrez-Gene
Orthologous residues
UBXN4 (human): T489‑p, UBXN4 (mouse): T487‑p, UBXN4 (rat): T487‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse

S695-p - UVRAG (mouse)
Modsite: sFRRPRRsSDK____ SwissProt Entrez-Gene
Orthologous residues
UVRAG (human): S696‑p, UVRAG (mouse): S695‑p, UVRAG (rat): S695‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  C2C12-D (muscle cell)
Cellular systems studied:  cell lines
Species studied:  mouse