Curated Information
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Home > Curated Information Page > Cst Curation Set #5168
Stokes M (2008) CST Curation Set: 5168; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T645-p - C2 (mouse)
Modsite: LRTGPEWtRCIQAVS SwissProt Entrez-Gene
Orthologous residues
C2 (human): T636‑p, C2 iso2 (human): T422‑p, C2 (mouse): T645‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S28-p - desmin (mouse)
Modsite: APGFsLGsPLssPVF SwissProt Entrez-Gene
Orthologous residues
desmin (human): S28‑p, desmin (mouse): S28‑p, desmin (rat): S28‑p, desmin (chicken): , desmin (dog): S28‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S31-p - desmin (mouse)
Modsite: FsLGsPLssPVFPrA SwissProt Entrez-Gene
Orthologous residues
desmin (human): S31‑p, desmin (mouse): S31‑p, desmin (rat): S31‑p, desmin (chicken): T21‑p, desmin (dog): G31‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S32-p - desmin (mouse)
Modsite: sLGsPLssPVFPrAG SwissProt Entrez-Gene
Orthologous residues
desmin (human): S32‑p, desmin (mouse): S32‑p, desmin (rat): S32‑p, desmin (chicken): S22‑p, desmin (dog): S32‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S455-p - DMAP1 (mouse)
Modsite: NsRKRREsAssSSSV SwissProt Entrez-Gene
Orthologous residues
DMAP1 (human): S454‑p, DMAP1 (mouse): S455‑p, DMAP1 (rat): S455‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S418-p - E1B-AP5 (mouse)
Modsite: RGTIGPKsKAECEIL SwissProt Entrez-Gene
Orthologous residues
E1B‑AP5 (human): S417‑p, E1B‑AP5 iso2 (human): S417‑p, E1B‑AP5 (mouse): S418‑p, E1B‑AP5 (rat): S418‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

T234-p - HS3ST3A1 (mouse)
Modsite: VVVRDPVtRAISDYT SwissProt Entrez-Gene
Orthologous residues
HS3ST3A1 (human): T247‑p, HS3ST3A1 (mouse): T234‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

T232-p - HS3ST3B1 (mouse)
Modsite: VVVRDPVtRAISDYT SwissProt Entrez-Gene
Orthologous residues
HS3ST3B1 (human): T232‑p, HS3ST3B1 (mouse): T232‑p, HS3ST3B1 (rat): T232‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S232-p - HTRA3 (mouse)
Modsite: IQDIDKKsDIATIVI SwissProt Entrez-Gene
Orthologous residues
HTRA3 (human): S226‑p, HTRA3 (mouse): S232‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S219-p - HYLS1 (mouse)
Modsite: LPRLDQLsRNRGKID SwissProt Entrez-Gene
Orthologous residues
HYLS1 (human): S204‑p, HYLS1 (mouse): S219‑p, HYLS1 (rat): S218‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

T84-p - MRPS30 (mouse)
Modsite: DEKIRILtKMQFKKY SwissProt Entrez-Gene
Orthologous residues
MRPS30 (human): T84‑p, MRPS30 (mouse): T84‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S116-p - PEA-15 (mouse)
Modsite: KDIIRQPsEEEIIKL SwissProt Entrez-Gene
Orthologous residues
PEA‑15 (human): S116‑p, PEA‑15 iso2 (human): S137‑p, PEA‑15 (mouse): S116‑p, PEA‑15 (rat): S116‑p, PEA‑15 (hamster): S116‑p, PEA‑15 (cow): S116‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S16-p - PLB (mouse)
Modsite: RSAIRRAstIEMPQQ SwissProt Entrez-Gene
Orthologous residues
PLB (human): S16‑p, PLB (mouse): S16‑p, PLB (rat): S16‑p, PLB (rabbit): S16‑p, PLB (pig): S16‑p, PLB (dog): S16‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

T17-p - PLB (mouse)
Modsite: SAIRRAstIEMPQQA SwissProt Entrez-Gene
Orthologous residues
PLB (human): T17‑p, PLB (mouse): T17‑p, PLB (rat): T17‑p, PLB (rabbit): T17‑p, PLB (pig): T17‑p, PLB (dog): T17‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S789-p - RALGAPB (mouse)
Modsite: KVKVMVDsGDRKRAI SwissProt Entrez-Gene
Orthologous residues
RALGAPB (human): S800‑p, RALGAPB iso2 (human): S578‑p, RALGAPB (mouse): S789‑p, RALGAPB iso3 (mouse): S800‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food

S307-p - Titin (mouse)
Modsite: PsPVrsVsPAGrIsT SwissProt Entrez-Gene
Orthologous residues
Titin (human): S308‑p, Titin iso3 (human): S308‑p, Titin iso6 (human): S308‑p, Titin iso12 (human): S308‑p, Titin (mouse): S307‑p, Titin iso3 (mouse): S307‑p, Titin iso4 (mouse): S307‑p, Titin (rat): S307‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  hypoxia with normal food