Curated Information
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Home > Curated Information Page > Cst Curation Set #4916
Guo A (2008) CST Curation Set: 4916; Year: 2008; Biosample/Treatment: tissue, muscle/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T297-p - ACTB (mouse)
Modsite: RkDLyANtVLsGGTT SwissProt Entrez-Gene
Orthologous residues
ACTB (human): T297‑p, ACTB (mouse): T297‑p, ACTB (rat): T297‑p, ACTB (cow): T297‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T297-p - ACTG1 (mouse)
Modsite: RkDLyANtVLsGGTT SwissProt Entrez-Gene
Orthologous residues
ACTG1 (human): T297‑p, ACTG1 (mouse): T297‑p, ACTG1 (rat): T297‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S3044-p - FLG (mouse)
Modsite: RGQsPDAsGRSSNRR SwissProt Entrez-Gene
Orthologous residues
FLG (human): S2851‑p, FLG (mouse): S3044‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T330-p - NDRG2 (mouse)
Modsite: tRLsRsRtAsLtsAA SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): T330‑p, NDRG2 iso2 (human): T316‑p, NDRG2 (mouse): T330‑p, NDRG2 (rat): T330‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S332-p - NDRG2 (mouse)
Modsite: LsRsRtAsLtsAAsI SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S332‑p, NDRG2 iso2 (human): S318‑p, NDRG2 (mouse): S332‑p, NDRG2 (rat): S332‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S338-p - NDRG2 (mouse)
Modsite: AsLtsAAsIDGsRsR SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S338‑p, NDRG2 iso2 (human): S324‑p, NDRG2 (mouse): S338‑p, NDRG2 (rat): S338‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S344-p - NDRG2 (mouse)
Modsite: AsIDGsRsRsRtLsQ SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S344‑p, NDRG2 iso2 (human): S330‑p, NDRG2 (mouse): S344‑p, NDRG2 (rat): S344‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T348-p - NDRG2 (mouse)
Modsite: GsRsRsRtLsQssEs SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): T348‑p, NDRG2 iso2 (human): T334‑p, NDRG2 (mouse): T348‑p, NDRG2 (rat): T348‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S350-p - NDRG2 (mouse)
Modsite: RsRsRtLsQssEsGt SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S350‑p, NDRG2 iso2 (human): S336‑p, NDRG2 (mouse): S350‑p, NDRG2 (rat): S350‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S352-p - NDRG2 (mouse)
Modsite: RsRtLsQssEsGtLP SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S352‑p, NDRG2 iso2 (human): S338‑p, NDRG2 (mouse): S352‑p, NDRG2 (rat): S352‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S353-p - NDRG2 (mouse)
Modsite: sRtLsQssEsGtLPs SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S353‑p, NDRG2 iso2 (human): S339‑p, NDRG2 (mouse): S353‑p, NDRG2 (rat): S353‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S23798-p - Titin (mouse)
Modsite: ATNCHKTsWKVDQLQ SwissProt Entrez-Gene
Orthologous residues
Titin (human): S22936‑p, Titin iso3 (human): S15512‑p, Titin iso6 (human): , Titin iso12 (human): S24577‑p, Titin (mouse): S23798‑p, Titin iso3 (mouse): , Titin iso4 (mouse): S22052‑p, Titin (rat): S22849‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T33859-p - Titin (mouse)
Modsite: VRPARRRtPsPDyDL SwissProt Entrez-Gene
Orthologous residues
Titin (human): T32998‑p, Titin iso3 (human): T25574‑p, Titin iso6 (human): , Titin iso12 (human): T34639‑p, Titin (mouse): T33859‑p, Titin iso3 (mouse): , Titin iso4 (mouse): T32113‑p, Titin (rat): T32847‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S33861-p - Titin (mouse)
Modsite: PARRRtPsPDyDLyy SwissProt Entrez-Gene
Orthologous residues
Titin (human): S33000‑p, Titin iso3 (human): S25576‑p, Titin iso6 (human): , Titin iso12 (human): S34641‑p, Titin (mouse): S33861‑p, Titin iso3 (mouse): , Titin iso4 (mouse): S32115‑p, Titin (rat): S32849‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S33933-p - Titin (mouse)
Modsite: LssLRyssPPAHVKV SwissProt Entrez-Gene
Orthologous residues
Titin (human): S33071‑p, Titin iso3 (human): S25647‑p, Titin iso6 (human): , Titin iso12 (human): S34712‑p, Titin (mouse): S33933‑p, Titin iso3 (mouse): , Titin iso4 (mouse): S32187‑p, Titin (rat): S32921‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S94-p - YAP1 (mouse)
Modsite: KsHsRQAstDAGtAG SwissProt Entrez-Gene
Orthologous residues
YAP1 (human): S109‑p, YAP1 iso2 (human): S109‑p, YAP1 iso3 (human): S109‑p, YAP1 (mouse): S94‑p, YAP1 (rat): S94‑p, YAP1 iso3 (rat): S94‑p, YAP1 (sheep): S24‑p, YAP1 (cow): S37‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T95-p - YAP1 (mouse)
Modsite: sHsRQAstDAGtAGA SwissProt Entrez-Gene
Orthologous residues
YAP1 (human): T110‑p, YAP1 iso2 (human): T110‑p, YAP1 iso3 (human): T110‑p, YAP1 (mouse): T95‑p, YAP1 (rat): T95‑p, YAP1 iso3 (rat): T95‑p, YAP1 (sheep): T25‑p, YAP1 (cow): T38‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout