Curated Information
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Home > Curated Information Page > Cst Curation Set #4914
Guo A (2008) CST Curation Set: 4914; Year: 2008; Biosample/Treatment: tissue, muscle/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y279-p - GSK3A (mouse)
Modsite: RGEPNVsyICsRYYR SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

Y216-p - GSK3B (mouse)
Modsite: RGEPNVsyICsRYYR SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit): Y216‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S328-p - NDRG2 (mouse)
Modsite: CMtRLsRsRtAsLts SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S328‑p, NDRG2 iso2 (human): S314‑p, NDRG2 (mouse): S328‑p, NDRG2 (rat): S328‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T330-p - NDRG2 (mouse)
Modsite: tRLsRsRtAsLtsAA SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): T330‑p, NDRG2 iso2 (human): T316‑p, NDRG2 (mouse): T330‑p, NDRG2 (rat): T330‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S332-p - NDRG2 (mouse)
Modsite: LsRsRtAsLtsAAsI SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S332‑p, NDRG2 iso2 (human): S318‑p, NDRG2 (mouse): S332‑p, NDRG2 (rat): S332‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T334-p - NDRG2 (mouse)
Modsite: RsRtAsLtsAAsIDG SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): T334‑p, NDRG2 iso2 (human): T320‑p, NDRG2 (mouse): T334‑p, NDRG2 (rat): T334‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S338-p - NDRG2 (mouse)
Modsite: AsLtsAAsIDGsRsR SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S338‑p, NDRG2 iso2 (human): S324‑p, NDRG2 (mouse): S338‑p, NDRG2 (rat): S338‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S344-p - NDRG2 (mouse)
Modsite: AsIDGsRsRsRtLsQ SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S344‑p, NDRG2 iso2 (human): S330‑p, NDRG2 (mouse): S344‑p, NDRG2 (rat): S344‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T348-p - NDRG2 (mouse)
Modsite: GsRsRsRtLsQssEs SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): T348‑p, NDRG2 iso2 (human): T334‑p, NDRG2 (mouse): T348‑p, NDRG2 (rat): T348‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S350-p - NDRG2 (mouse)
Modsite: RsRsRtLsQssEsGt SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S350‑p, NDRG2 iso2 (human): S336‑p, NDRG2 (mouse): S350‑p, NDRG2 (rat): S350‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S352-p - NDRG2 (mouse)
Modsite: RsRtLsQssEsGtLP SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S352‑p, NDRG2 iso2 (human): S338‑p, NDRG2 (mouse): S352‑p, NDRG2 (rat): S352‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S353-p - NDRG2 (mouse)
Modsite: sRtLsQssEsGtLPs SwissProt Entrez-Gene
Orthologous residues
NDRG2 (human): S353‑p, NDRG2 iso2 (human): S339‑p, NDRG2 (mouse): S353‑p, NDRG2 (rat): S353‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

Y32052-p - Titin (mouse)
Modsite: GGADILGyILERREV SwissProt Entrez-Gene
Orthologous residues
Titin (human): Y31190‑p, Titin iso3 (human): Y23766‑p, Titin iso6 (human): , Titin iso12 (human): Y32831‑p, Titin (mouse): Y32052‑p, Titin iso3 (mouse): , Titin iso4 (mouse): Y30306‑p, Titin (rat): Y31040‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

T33859-p - Titin (mouse)
Modsite: VRPARRRtPsPDyDL SwissProt Entrez-Gene
Orthologous residues
Titin (human): T32998‑p, Titin iso3 (human): T25574‑p, Titin iso6 (human): , Titin iso12 (human): T34639‑p, Titin (mouse): T33859‑p, Titin iso3 (mouse): , Titin iso4 (mouse): T32113‑p, Titin (rat): T32847‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S33861-p - Titin (mouse)
Modsite: PARRRtPsPDyDLyy SwissProt Entrez-Gene
Orthologous residues
Titin (human): S33000‑p, Titin iso3 (human): S25576‑p, Titin iso6 (human): , Titin iso12 (human): S34641‑p, Titin (mouse): S33861‑p, Titin iso3 (mouse): , Titin iso4 (mouse): S32115‑p, Titin (rat): S32849‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout

S33933-p - Titin (mouse)
Modsite: LssLRyssPPAHVKV SwissProt Entrez-Gene
Orthologous residues
Titin (human): S33071‑p, Titin iso3 (human): S25647‑p, Titin iso6 (human): , Titin iso12 (human): S34712‑p, Titin (mouse): S33933‑p, Titin iso3 (mouse): , Titin iso4 (mouse): S32187‑p, Titin (rat): S32921‑p, Titin iso2 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  muscle [Akt2 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Akt2 -/- knockout