Curated Information
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Home > Curated Information Page > PubMed Id: 17579208
Lim MJ, et al. (2007) RhoA/Rho kinase blocks muscle differentiation via serine phosphorylation of insulin receptor substrate-1 and -2. Mol Endocrinol 21, 2282-93 17579208
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S302-p - IRS1 (mouse)
Modsite: TRRSRtEsITAtsPA SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S307‑p, IRS1 (mouse): S302‑p, IRS1 (rat): S302‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), H9c2 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (rat) pharmacological inhibitor of upstream enzyme, phospho-antibody, co-immunoprecipitation, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_withdrawal decrease differentiation media
FGF2 serum_withdrawal inhibit treatment-induced decrease
Y27632 FGF2 inhibit treatment-induced increase

S632-p - IRS1 (mouse)
Modsite: NGDyMPMsPKsVsAP SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S636‑p, IRS1 (mouse): S632‑p, IRS1 (rat): S632‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), H9c2 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (rat) pharmacological inhibitor of upstream enzyme, phospho-antibody, co-immunoprecipitation, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_withdrawal decrease differentiation media

S635-p - IRS1 (mouse)
Modsite: yMPMsPKsVsAPQQI SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S639‑p, IRS1 (mouse): S635‑p, IRS1 (rat): S635‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), H9c2 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (rat) pharmacological inhibitor of upstream enzyme, phospho-antibody, co-immunoprecipitation, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_withdrawal decrease differentiation media

S302-p - IRS1 (rat)
Modsite: TRRSRTEsITATsPA SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S307‑p, IRS1 (mouse): S302‑p, IRS1 (rat): S302‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), H9c2 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (rat) pharmacological inhibitor of upstream enzyme, phospho-antibody, co-immunoprecipitation, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_withdrawal decrease differentiation media
Y27632 decrease

S632-p - IRS1 (rat)
Modsite: NGDyMPMsPKsVsAP SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S636‑p, IRS1 (mouse): S632‑p, IRS1 (rat): S632‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), H9c2 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (rat) pharmacological inhibitor of upstream enzyme, phospho-antibody, co-immunoprecipitation, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_withdrawal decrease differentiation media
Y27632 decrease

S635-p - IRS1 (rat)
Modsite: yMPMsPKsVsAPQQI SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S639‑p, IRS1 (mouse): S635‑p, IRS1 (rat): S635‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), H9c2 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (rat) pharmacological inhibitor of upstream enzyme, phospho-antibody, co-immunoprecipitation, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_withdrawal decrease differentiation media
Y27632 decrease

T697-p - MYPT1 (rat)
Modsite: ARQSRRstQGVTLTD SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T696‑p, MYPT1 iso2 (human): T696‑p, MYPT1 iso3 (human): T640‑p, MYPT1 iso4 (human): T637‑p, MYPT1 (mouse): T694‑p, MYPT1 (rat): T697‑p, MYPT1 (pig): T696‑p, MYPT1 (chicken): T695‑p, MYPT1 (cow): T696‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), H9c2 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum_withdrawal decrease differentiation media

T412-p - p70S6K (rat)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), H9c2 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Y27632 increase
serum_withdrawal increase differentiation media
Y27632 serum_withdrawal augment treatment-induced increase