Li H, et al. (2014) TC-PTP directly interacts with connexin43 to regulate gap junction intercellular communication. J Cell Sci 127, 3269-79 24849651

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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Y247-p - GJA1 (rat) ▲

Modsite: VKGRsDPyHATTGPL SwissProt Entrez-Gene Orthologous residues GJA1 (human): Y247‑p, GJA1 (mouse): Y247‑p, GJA1 (rat): Y247‑p, GJA1 (rabbit): Y247‑p, GJA1 (pig): Y247‑p, GJA1 (hamster): Y247‑p, GJA1 (cow): Y248‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  LA-25 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme PHOSPHATASE PTPN2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Src (human) activation of upstream enzyme PHOSPHATASE PTPN2 (human) co-immunoprecipitation, microscopy-colocalization Comments:  active form the enzyme Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PTPN2 (human) Induces pull-down assay, co-immunoprecipitation, NMR spectroscopy

Y265-p - GJA1 (rat) ▲

Modsite: kDCGsPkyAyFNGCS SwissProt Entrez-Gene Orthologous residues GJA1 (human): Y265‑p, GJA1 (mouse): Y265‑p, GJA1 (rat): Y265‑p, GJA1 (rabbit): Y265‑p, GJA1 (pig): Y265‑p, GJA1 (hamster): Y265‑p, GJA1 (cow): Y266‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  LA-25 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme PHOSPHATASE PTPN2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PTPN2 (human) co-immunoprecipitation, microscopy-colocalization KINASE Src (human) activation of upstream enzyme Comments:  active form the enzyme Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PTPN2 (human) Induces pull-down assay, co-immunoprecipitation, NMR spectroscopy

S279-p - GJA1 (rat) ▲

Modsite: SSPTAPLsPMsPPGY SwissProt Entrez-Gene Orthologous residues GJA1 (human): S279‑p, GJA1 (mouse): S279‑p, GJA1 (rat): S279‑p, GJA1 (rabbit): S279‑p, GJA1 (pig): S279‑p, GJA1 (hamster): S279‑p, GJA1 (cow): S280‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  LA-25 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U0126 decrease

S282-p - GJA1 (rat) ▲

Modsite: TAPLsPMsPPGYkLV SwissProt Entrez-Gene Orthologous residues GJA1 (human): S282‑p, GJA1 (mouse): S282‑p, GJA1 (rat): S282‑p, GJA1 (rabbit): S282‑p, GJA1 (pig): S282‑p, GJA1 (hamster): S282‑p, GJA1 (cow): S283‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  LA-25 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U0126 decrease

S368-p - GJA1 (rat) ▲

Modsite: QRPssRAssRAssRP SwissProt Entrez-Gene Orthologous residues GJA1 (human): S368‑p, GJA1 (mouse): S368‑p, GJA1 (rat): S368‑p, GJA1 (rabbit): S368‑p, GJA1 (pig): S368‑p, GJA1 (hamster): S368‑p, GJA1 (cow): S369‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  LA-25 (fibroblast) Cellular systems studied:  cell lines Species studied:  rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PTPN2 (human) co-immunoprecipitation, microscopy-colocalization