Curated Information
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Home > Curated Information Page > PubMed Id: 24813943
Del Re DP, et al. (2014) Mst1 Promotes Cardiac Myocyte Apoptosis through Phosphorylation and Inhibition of Bcl-xL. Mol Cell 54, 639-50 24813943
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S14-p - Bcl-xL (mouse)
Modsite: ELVVDFLsYKLSQKG SwissProt Entrez-Gene
Orthologous residues
Bcl‑xL (human): S14‑p, Bcl‑xL (mouse): S14‑p, Bcl‑xL (rat): S14‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), myocyte-heart
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MST1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MST1 (human) phospho-antibody, siRNA inhibition of enzyme shRNA
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
ischemia/reperfusion increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  apoptosis, induced, signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
BID (human) BH4 Induces co-immunoprecipitation
BAX (human) BH4 Disrupts co-immunoprecipitation, pull-down assay
BIM (human) BH4 Induces co-immunoprecipitation
Associated Diseases
Diseases Alterations Comments
cardiomyopathy increased

S62-p - Bcl-xL (mouse)
Modsite: PSWHLADsPAVNGAT SwissProt Entrez-Gene
Orthologous residues
Bcl‑xL (human): S62‑p, Bcl‑xL (mouse): S62‑p, Bcl‑xL (rat): S62‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia/reperfusion no change compared to control