Ponce-Coria J, et al. (2014) A Novel Ste20-related Proline/Alanine-rich Kinase (SPAK)-independent Pathway Involving Calcium-binding Protein 39 (Cab39) and Serine Threonine Kinase with No Lysine Member 4 (WNK4) in the Activation of Na-K-Cl Cotransporters. J Biol Chem 289, 17680-17688 24811174

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T196-p - NKCC1 (mouse) ▲

Modsite: HQQYYYDtHTNtYYL SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T203‑p, NKCC1 iso3 (human): T203‑p, NKCC1 (mouse): T196‑p, NKCC1 (rat): T194‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  oocyte Cellular systems studied:  primary cells Species studied:  frog Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WNK4 (mouse), CAB39 (mouse) increase

T200-p - NKCC1 (mouse) ▲

Modsite: YYDtHTNtYYLRtFG SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T207‑p, NKCC1 iso3 (human): T207‑p, NKCC1 (mouse): T200‑p, NKCC1 (rat): T198‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  oocyte Cellular systems studied:  primary cells Species studied:  frog Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WNK4 (mouse), CAB39 (mouse) increase

T205-p - NKCC1 (mouse) ▲

Modsite: TNtYYLRtFGHNtMD SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T212‑p, NKCC1 iso3 (human): T212‑p, NKCC1 (mouse): T205‑p, NKCC1 (rat): T203‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  oocyte Cellular systems studied:  primary cells Species studied:  frog Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WNK4 (mouse), CAB39 (mouse) increase