Curated Information
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Home > Curated Information Page > Cst Curation Set #3012
Possemato A (2007) CST Curation Set: 3012; Year: 2007; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S710-p - acinus (human)
Modsite: sVQARRLsQPEsAEk SwissProt Entrez-Gene
Orthologous residues
acinus (human): S710‑p, acinus iso2 (human): , acinus iso5 (human): S710‑p, acinus (mouse): S710‑p, acinus iso4 (mouse): S670‑p, acinus iso5 (mouse): , acinus (rat): S711‑p, acinus (cow): S703‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S89-p - CAAP1 (human)
Modsite: SERRKRRstDsssVs SwissProt Entrez-Gene
Orthologous residues
CAAP1 (human): S89‑p, CAAP1 (mouse): S68‑p, CAAP1 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T160-p - CDK2 (human)
Modsite: GVPVRtytHEVVtLW SwissProt Entrez-Gene
Orthologous residues
CDK2 (human): T160‑p, CDK2 iso2 (human): T160‑p, CDK2 (mouse): T160‑p, CDK2 (rat): T160‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T154-p - DOS iso4 (human)
Modsite: QDKGRRYtLTEGDFH SwissProt Entrez-Gene
Orthologous residues
DOS (human): T154‑p, DOS iso4 (human): T154‑p, DOS (mouse): T150‑p, DOS (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T54-p - DSCR2 (human)
Modsite: VRLLRRQtktsLEVS SwissProt Entrez-Gene
Orthologous residues
DSCR2 (human): T54‑p, DSCR2 iso2 (human): T54‑p, DSCR2 (mouse): T55‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T574-p - eIF3-theta (human)
Modsite: RILARRQtIEERKER SwissProt Entrez-Gene
Orthologous residues
eIF3‑theta (human): T574‑p, eIF3‑theta (mouse): T574‑p, eIF3‑theta (rat): T574‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S442-p - EIF4B (human)
Modsite: RNARRREsEksLENE SwissProt Entrez-Gene
Orthologous residues
EIF4B (human): S442‑p, EIF4B (mouse): S442‑p, EIF4B (rat): S442‑p, EIF4B (cow): S442‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T328-p - FMIP (human)
Modsite: tTKRRRPtLGVQLDD SwissProt Entrez-Gene
Orthologous residues
FMIP (human): T328‑p, FMIP (mouse): T328‑p, FMIP (rat): T327‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S784-p - GTF2I (human)
Modsite: GVPFRRPstFGIPRL SwissProt Entrez-Gene
Orthologous residues
GTF2I (human): S784‑p, GTF2I iso2 (human): S743‑p, GTF2I iso4 (human): S763‑p, GTF2I (mouse): S784‑p, GTF2I iso8 (mouse): S744‑p, GTF2I (rat): S765‑p, GTF2I iso2 (rat): S699‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T1305-p - HBXAP (human)
Modsite: KRLHRIEtDEEEsCD SwissProt Entrez-Gene
Orthologous residues
HBXAP (human): T1305‑p, HBXAP iso2 (human): T1274‑p, HBXAP iso3 (human): T1053‑p, HBXAP (mouse): T1308‑p, HBXAP (rat): T1313‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S1764-p - HELZ (human)
Modsite: LyQRRIssssVQPCS SwissProt Entrez-Gene
Orthologous residues
HELZ (human): S1764‑p, HELZ (mouse): S1786‑p, HELZ (rat): S1786‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S87-p - HN1 (human)
Modsite: sGLQRRNssEAssGD SwissProt Entrez-Gene
Orthologous residues
HN1 (human): S87‑p, HN1 iso2 (human): S87‑p, HN1 (mouse): S87‑p, HN1 (rat): S82‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S26-p - hnRNP U (human)
Modsite: ELkKRRLsDkGLkAE SwissProt Entrez-Gene
Orthologous residues
hnRNP U (human): S26‑p, hnRNP U iso2 (human): S26‑p, hnRNP U (mouse): S26‑p, hnRNP U (rat): S26‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S902-p - KIF23 (human)
Modsite: IEtLkQEsPNGsRKR SwissProt Entrez-Gene
Orthologous residues
KIF23 (human): S902‑p, KIF23 iso2 (human): S798‑p, KIF23 iso3 (human): S615‑p, KIF23 (mouse): L895‑p, KIF23 (rat): S894‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S911-p - KIF23 (human)
Modsite: NGsRKRRsstVAPAQ SwissProt Entrez-Gene
Orthologous residues
KIF23 (human): S911‑p, KIF23 iso2 (human): S807‑p, KIF23 iso3 (human): S624‑p, KIF23 (mouse): S904‑p, KIF23 (rat): S903‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S912-p - KIF23 (human)
Modsite: GsRKRRsstVAPAQP SwissProt Entrez-Gene
Orthologous residues
KIF23 (human): S912‑p, KIF23 iso2 (human): S808‑p, KIF23 iso3 (human): S625‑p, KIF23 (mouse): S905‑p, KIF23 (rat): S904‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T799-p - KIF4A (human)
Modsite: PPKLRRRtFsLTEVR SwissProt Entrez-Gene
Orthologous residues
KIF4A (human): T799‑p, KIF4A iso2 (human): T799‑p, KIF4A (mouse): T800‑p, KIF4A (rat): T800‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T507-p - MARK3 (human)
Modsite: GGMtRRNtyVCsERT SwissProt Entrez-Gene
Orthologous residues
MARK3 (human): T507‑p, MARK3 iso3 (human): T507‑p, MARK3 iso6 (human): T491‑p, MARK3 (mouse): T507‑p, MARK3 (rat): T507‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S188-p - matrin 3 (human)
Modsite: kRHFRRDsFDDrGPs SwissProt Entrez-Gene
Orthologous residues
matrin 3 (human): S188‑p, matrin 3 iso2 (human): , matrin 3 iso3 (human): S188‑p, matrin 3 (mouse): S188‑p, matrin 3 (rat): S188‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S546-p - MORC3 (human)
Modsite: NsLkRRLstRssILN SwissProt Entrez-Gene
Orthologous residues
MORC3 (human): S546‑p, MORC3 (mouse): G549‑p, MORC3 (rat): S286‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S560-p - MORC3 (human)
Modsite: NAkNRRLssQFENSV SwissProt Entrez-Gene
Orthologous residues
MORC3 (human): S560‑p, MORC3 (mouse): S563‑p, MORC3 (rat): S300‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S561-p - MORC3 (human)
Modsite: AkNRRLssQFENSVY SwissProt Entrez-Gene
Orthologous residues
MORC3 (human): S561‑p, MORC3 (mouse): N564‑p, MORC3 (rat): N301‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T542-p - MPRIP (human)
Modsite: RREGRsKtFDWAEFR SwissProt Entrez-Gene
Orthologous residues
MPRIP (human): T542‑p, MPRIP iso2 (human): T542‑p, MPRIP iso3 (human): T504‑p, MPRIP (mouse): T540‑p, MPRIP iso6 (mouse): T504‑p, MPRIP (rat): T545‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T21-p - NCBP1 (human)
Modsite: QPHKRRKtsDANEtE SwissProt Entrez-Gene
Orthologous residues
NCBP1 (human): T21‑p, NCBP1 (mouse): T21‑p, NCBP1 (rat): T21‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T624-p - PHACTR4 (human)
Modsite: REIKRRLtRKLsQRP SwissProt Entrez-Gene
Orthologous residues
PHACTR4 (human): T624‑p, PHACTR4 iso2 (human): T634‑p, PHACTR4 (mouse): T616‑p, PHACTR4 (rat): T632‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S628-p - PHACTR4 (human)
Modsite: RRLtRKLsQRPtVAE SwissProt Entrez-Gene
Orthologous residues
PHACTR4 (human): S628‑p, PHACTR4 iso2 (human): S638‑p, PHACTR4 (mouse): S620‑p, PHACTR4 (rat): S636‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S114-p - PKAR2B (human)
Modsite: NRFTRRAsVCAEAyN SwissProt Entrez-Gene
Orthologous residues
PKAR2B (human): S114‑p, PKAR2B (mouse): S112‑p, PKAR2B (rat): S112‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T484-p - QIK (human)
Modsite: RSGQRRHtLsEVTNQ SwissProt Entrez-Gene
Orthologous residues
QIK (human): T484‑p, QIK (mouse): T484‑p, QIK (rat): T484‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T1071-p - RAPGEF1 (human)
Modsite: RNItRRKtDREEKt_ SwissProt Entrez-Gene
Orthologous residues
RAPGEF1 (human): T1071‑p, RAPGEF1 iso2 (human): T1032‑p, RAPGEF1 (mouse): T1080‑p, RAPGEF1 iso2 (mouse): T1212‑p, RAPGEF1 (rat): T1007‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S272-p - REPS1 (human)
Modsite: AIEIRRQsssyDDPW SwissProt Entrez-Gene
Orthologous residues
REPS1 (human): S272‑p, REPS1 iso2 (human): S272‑p, REPS1 iso3 (human): S272‑p, REPS1 iso4 (human): S272‑p, REPS1 (mouse): S272‑p, REPS1 iso3 (mouse): S220‑p, REPS1 (rat): S272‑p, REPS1 iso4 (rat):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S235-p - S6 (human)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S236-p - S6 (human)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S240-p - S6 (human)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T341-p - sialophorin (human)
Modsite: EGssRRPtLttFFGR SwissProt Entrez-Gene
Orthologous residues
sialophorin (human): T341‑p, sialophorin (mouse): T333‑p, sialophorin (rat): T316‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S351-p - sialophorin (human)
Modsite: tFFGRRKsRQGsLAM SwissProt Entrez-Gene
Orthologous residues
sialophorin (human): S351‑p, sialophorin (mouse): S343‑p, sialophorin (rat): S326‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S355-p - sialophorin (human)
Modsite: RRKsRQGsLAMEELk SwissProt Entrez-Gene
Orthologous residues
sialophorin (human): S355‑p, sialophorin (mouse): S347‑p, sialophorin (rat): S330‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S72-p - SNX3 (human)
Modsite: StVRRRysDFEWLRs SwissProt Entrez-Gene
Orthologous residues
SNX3 (human): S72‑p, SNX3 iso2 (human): , SNX3 iso3 (human): S72‑p, SNX3 iso4 (human): S50‑p, SNX3 (mouse): S72‑p, SNX3 (rat): S72‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S492-p - SRGAP2 (human)
Modsite: DCsLARRsstVRKQD SwissProt Entrez-Gene
Orthologous residues
SRGAP2 (human): S492‑p, SRGAP2 iso2 (human): S492‑p, SRGAP2 (mouse): S492‑p, SRGAP2 (rat): S492‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

S493-p - SRGAP2 (human)
Modsite: CsLARRsstVRKQDs SwissProt Entrez-Gene
Orthologous residues
SRGAP2 (human): S493‑p, SRGAP2 iso2 (human): S493‑p, SRGAP2 (mouse): S493‑p, SRGAP2 (rat): S493‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human

T42-p - TBC1D5 (human)
Modsite: sNKNGRRtsstLDsE SwissProt Entrez-Gene
Orthologous residues
TBC1D5 (human): T42‑p, TBC1D5 iso2 (human): T42‑p, TBC1D5 (mouse): S42‑p, TBC1D5 (rat): A42‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  K562 (erythroid)
Cellular systems studied:  cell lines
Species studied:  human