Curated Information
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Home > Curated Information Page > PubMed Id: 24769357
Girardi C, et al. (2014) Differential phosphorylation of Akt1 and Akt2 by protein kinase CK2 may account for isoform specific functions. Biochim Biophys Acta 1843, 1865-74 24769357
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S129-p - Akt1 (human)
Modsite: sGsPsDNsGAEEMEV SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S129‑p, Akt1 iso2 (human): S67‑p, Akt1 (mouse): S129‑p, Akt1 (rat): S129‑p, Akt1 (fruit fly): E241‑p, Akt1 (cow): S129‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast), HEK293T (epithelial) [Akt1 (human), transfection], HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
okadaic_acid no change compared to control
Akt1 (human) increase Akt1 siRNA decrease
Akt2 (human) no change compared to control Akt2 siRNA no change
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
palladin (human) Induces co-immunoprecipitation

S126-p - Akt2 (human)
Modsite: PMDyKCGsPsDSsTT SwissProt Entrez-Gene
Orthologous residues
Akt2 (human): S126‑p, Akt2 (mouse): S126‑p, Akt2 (rat): S126‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S128-p - Akt2 (human)
Modsite: DyKCGsPsDSsTTEE SwissProt Entrez-Gene
Orthologous residues
Akt2 (human): S128‑p, Akt2 (mouse): S128‑p, Akt2 (rat): S128‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S131-p - Akt2 (human)
Modsite: CGsPsDSsTTEEMEV SwissProt Entrez-Gene
Orthologous residues
Akt2 (human): S131‑p, Akt2 (mouse): S131‑p, Akt2 (rat): S131‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial) [Akt2 (human), transfection], HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK2A1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
okadaic_acid no change compared to control