Shenberger JS, et al. (2007) Roles of mitogen-activated protein kinase signal-integrating kinases 1 and 2 in oxidant-mediated eIF4E phosphorylation. Int J Biochem Cell Biol 39, 1828-42 17689282

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S209-p - EIF4E (human) ▲

Modsite: DtATksGsttKNRFV SwissProt Entrez-Gene Orthologous residues EIF4E (human): S209‑p, EIF4E iso2 (human): S240‑p, EIF4E (mouse): S209‑p, EIF4E (rat): S209‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  A549 (pulmonary), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Mnk1 (mouse) genetic knockout/knockin of upstream enzyme, phospho-antibody, pharmacological activator of upstream enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase CGP57380 H2O2 inhibit treatment-induced increase

T250-p - Mnk1 (human) ▲

Modsite: NSCTPITtPELTtPC SwissProt Entrez-Gene Orthologous residues Mnk1 (human): T250‑p, Mnk1 iso2 (human): T209‑p, Mnk1 (mouse): T209‑p, Mnk1 (rat): T197‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  A549 (pulmonary), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase CGP57380 increase H2O2 CGP57380 augment treatment-induced increase

T255-p - Mnk1 (human) ▲

Modsite: ITtPELTtPCGSAEY SwissProt Entrez-Gene Orthologous residues Mnk1 (human): T255‑p, Mnk1 iso2 (human): T214‑p, Mnk1 (mouse): T214‑p, Mnk1 (rat): T202‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  A549 (pulmonary), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase CGP57380 increase H2O2 CGP57380 augment treatment-induced increase

S209-p - EIF4E (mouse) ▲

Modsite: DTATKSGsttKNRFV SwissProt Entrez-Gene Orthologous residues EIF4E (human): S209‑p, EIF4E iso2 (human): S240‑p, EIF4E (mouse): S209‑p, EIF4E (rat): S209‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  A549 (pulmonary), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Mnk1 (mouse) genetic knockout/knockin of upstream enzyme, phospho-antibody, pharmacological activator of upstream enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase H2O2 Mnk1 (mouse) inhibit treatment-induced increase knockout H2O2 Mnk2 (mouse) inhibit treatment-induced increase knockout (slight effect) Mnk1 (mouse) Mnk2 (mouse) augment treatment-induced decrease knockout

T180-p - P38A (mouse) ▲

Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene Orthologous residues P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  A549 (pulmonary), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase H2O2 Mnk1 (mouse) no effect upon treatment-induced increase knockout H2O2 Mnk2 (mouse) inhibit treatment-induced increase knockout

Y182-p - P38A (mouse) ▲

Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene Orthologous residues P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  lung cancer Relevant cell lines - cell types - tissues:  A549 (pulmonary), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes H2O2 increase H2O2 Mnk1 (mouse) no effect upon treatment-induced increase knockout H2O2 Mnk2 (mouse) inhibit treatment-induced increase knockout