Curated Information
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Home > Curated Information Page > PubMed Id: 24760147
Wu Z, et al. (2014) Calpain-1 contributes to IgE-mediated mast cell activation. J Immunol 192, 5130-9 24760147
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BMMC (mast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BMMC (mast)
Cellular systems studied:  cell lines
Species studied:  mouse

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BMMC (mast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BMMC (mast)
Cellular systems studied:  cell lines
Species studied:  mouse

S180-p - IKKA (mouse)
Modsite: DQGsLCTsFVGTLQY SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S180‑p, IKKA (mouse): S180‑p, IKKA (rat): S180‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BMMC (mast)
Cellular systems studied:  cell lines
Species studied:  mouse

S181-p - IKKB (mouse)
Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BMMC (mast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNP increase
TNP CAPN1 (mouse) increase CAPN1 -/- decrease

S95-p - SNAP23 (mouse)
Modsite: NRTkNFEsGKNYkAt SwissProt Entrez-Gene
Orthologous residues
SNAP23 (human): S95‑p, SNAP23 (mouse): S95‑p, SNAP23 (rat): S95‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNP increase
TNP CAPN1 (mouse) increase CAPN1 -/- decrease

S120-p - SNAP23 (mouse)
Modsite: NVVskQPsRITNGQP SwissProt Entrez-Gene
Orthologous residues
SNAP23 (human): G120‑p, SNAP23 (mouse): S120‑p, SNAP23 (rat): S120‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BMMC (mast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNP increase
TNP CAPN1 (mouse) increase CAPN1 -/- decrease