Curated Information
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Home > Curated Information Page > PubMed Id: 29162720
Ji W, Luo Y, Ahmad E, Liu ST (2018) Direct interactions of mitotic arrest deficient 1 (MAD1) domains with each other and MAD2 conformers are required for mitotic checkpoint signaling. J Biol Chem 293, 484-496 29162720
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T8-p - MAD1L1 (human)
Modsite: MEDLGENtMVLstLR SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): T8‑p, MAD1L1 (mouse): T8‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S22-p - MAD1L1 (human)
Modsite: RsLNNFIsQRVEGGs SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S22‑p, MAD1L1 (mouse): S22‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S62-p - MAD1L1 (human)
Modsite: AEQIRSksHLIQVER SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S62‑p, MAD1L1 (mouse): S62‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

T323-p - MAD1L1 (human)
Modsite: SWERLDQtMGLSIRT SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): T323‑p, MAD1L1 (mouse): T323‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S598-p - MAD1L1 (human)
Modsite: AAASLPsskEVAELK SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S598‑p, MAD1L1 (mouse): S597‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Mad2 (human) Induces co-immunoprecipitation

S610-p - MAD1L1 (human)
Modsite: ELKKQVEsAELkNQR SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): S610‑p, MAD1L1 (mouse): S609‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Mad2 (human) Induces co-immunoprecipitation

T624-p - MAD1L1 (human)
Modsite: RLkEVFQtkIQEFRK SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): T624‑p, MAD1L1 (mouse): T623‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Mad2 (human) Induces co-immunoprecipitation

Y634-p - MAD1L1 (human)
Modsite: QEFRKACyTLTGYQI SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): Y634‑p, MAD1L1 (mouse): Y633‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human

T716-p - MAD1L1 (human)
Modsite: LELFSRQtVA_____ SwissProt Entrez-Gene
Orthologous residues
MAD1L1 (human): T716‑p, MAD1L1 (mouse): T715‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), SF9
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Mad2 (human) Induces co-immunoprecipitation