Curated Information
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Home > Curated Information Page > PubMed Id: 29141220
Dushukyan N, et al. (2017) Phosphorylation and Ubiquitination Regulate Protein Phosphatase 5 Activity and Its Prosurvival Role in Kidney Cancer. Cell Rep 21, 1883-1895 29141220
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S13-p - CDC37 (human)
Modsite: VWDHIEVsDDEDETH SwissProt Entrez-Gene
Orthologous residues
CDC37 (human): S13‑p, CDC37 (mouse): S13‑p, CDC37 (rat): S13‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  kidney cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), 786-O (renal), A498 (renal), Caki-1 (renal), E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP5C (human)

K185-ub - PPP5C (human)
Modsite: GPKLEDGkVTISFMK SwissProt Entrez-Gene
Orthologous residues
PPP5C (human): K185‑ub, PPP5C (mouse): K185‑ub, PPP5C (rat): K185‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  kidney cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), 786-O (renal), A498 (renal), Caki-1 (renal), E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE VHL (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE VHL (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  protein degradation, ubiquitination

K199-ub - PPP5C (human)
Modsite: KELMQWYkDQKKLHR SwissProt Entrez-Gene
Orthologous residues
PPP5C (human): K199‑ub, PPP5C (mouse): K199‑ub, PPP5C (rat): K199‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  kidney cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), 786-O (renal), A498 (renal), Caki-1 (renal), E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE VHL (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE VHL (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  protein degradation, ubiquitination

T362-p - PPP5C (human)
Modsite: LFSEDGVtLDDIRKI SwissProt Entrez-Gene
Orthologous residues
PPP5C (human): T362‑p, PPP5C (mouse): T362‑p, PPP5C (rat): T362‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  kidney cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), 786-O (renal), A498 (renal), Caki-1 (renal), E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1D (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1D (human) modification site within consensus motif, transfection of wild-type enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  apoptosis, inhibited, cell growth, induced