Dushukyan N, et al. (2017) Phosphorylation and Ubiquitination Regulate Protein Phosphatase 5 Activity and Its Prosurvival Role in Kidney Cancer. Cell Rep 21, 1883-1895 29141220

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S13-p - CDC37 (human) ▲

Modsite: VWDHIEVsDDEDETH SwissProt Entrez-Gene Orthologous residues CDC37 (human): S13‑p, CDC37 (mouse): S13‑p, CDC37 (rat): S13‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  kidney cancer Relevant cell lines - cell types - tissues:  293 (epithelial), 786-O (renal), A498 (renal), Caki-1 (renal), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme PHOSPHATASE PPP5C (human)

K185-ub - PPP5C (human) ▲

Modsite: GPKLEDGkVTISFMK SwissProt Entrez-Gene Orthologous residues PPP5C (human): K185‑ub, PPP5C (mouse): K185‑ub, PPP5C (rat): K185‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  kidney cancer Relevant cell lines - cell types - tissues:  293 (epithelial), 786-O (renal), A498 (renal), Caki-1 (renal), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE VHL (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE VHL (human) transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination

K199-ub - PPP5C (human) ▲

Modsite: KELMQWYkDQKKLHR SwissProt Entrez-Gene Orthologous residues PPP5C (human): K199‑ub, PPP5C (mouse): K199‑ub, PPP5C (rat): K199‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  kidney cancer Relevant cell lines - cell types - tissues:  293 (epithelial), 786-O (renal), A498 (renal), Caki-1 (renal), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE VHL (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE VHL (human) transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination

T362-p - PPP5C (human) ▲

Modsite: LFSEDGVtLDDIRKI SwissProt Entrez-Gene Orthologous residues PPP5C (human): T362‑p, PPP5C (mouse): T362‑p, PPP5C (rat): T362‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  kidney cancer Relevant cell lines - cell types - tissues:  293 (epithelial), 786-O (renal), A498 (renal), Caki-1 (renal), E.coli (bacterial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CK1D (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CK1D (human) co-immunoprecipitation, transfection of wild-type enzyme, modification site within consensus motif Downstream Regulation Effect of modification (function):  enzymatic activity, induced Effect of modification (process):  apoptosis, inhibited, cell growth, induced