Curated Information
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Home > Curated Information Page > PubMed Id: 29133485
Genna A, et al. (2018) Pyk2 and FAK differentially regulate invadopodia formation and function in breast cancer cells. J Cell Biol 217, 375-395 29133485
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y421-p - Cortactin (human)
Modsite: rLPssPVyEDAAsFK SwissProt Entrez-Gene
Orthologous residues
Cortactin (human): Y421‑p, Cortactin iso2 (human): Y384‑p, Cortactin iso3 (human): Y384‑p, Cortactin (mouse): Y421‑p, Cortactin (rat): Y383‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pyk2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) co-immunoprecipitation, microscopy-colocalization, siRNA inhibition of enzyme recruitment and activation of Src kinase

Y470-p - Cortactin (human)
Modsite: AyATEAVyESAEAPG SwissProt Entrez-Gene
Orthologous residues
Cortactin (human): Y470‑p, Cortactin iso2 (human): Y433‑p, Cortactin iso3 (human): Y433‑p, Cortactin (mouse): Y466‑p, Cortactin (rat): Y428‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pyk2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) co-immunoprecipitation, microscopy-colocalization, siRNA inhibition of enzyme recruitment and activation of Src kinase

Y486-p - Cortactin (human)
Modsite: yPAEDStyDEYENDL SwissProt Entrez-Gene
Orthologous residues
Cortactin (human): Y486‑p, Cortactin iso2 (human): Y449‑p, Cortactin iso3 (human): Y449‑p, Cortactin (mouse): Y482‑p, Cortactin (rat): Y444‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Pyk2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Pyk2 (human) co-immunoprecipitation, microscopy-colocalization, siRNA inhibition of enzyme recruitment and activation of Src kinase

Y397-p - FAK (human)
Modsite: sVsEtDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MDA-MB-231 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced