Curated Information
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Home > Curated Information Page > PubMed Id: 29133416
Yao K, et al. (2017) RSK2 phosphorylates T-bet to attenuate colon cancer metastasis and growth. Proc Natl Acad Sci U S A 114, 12791-12796 29133416
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S503-p - TBX21 (human)
Modsite: DSKRRRVsPYPsSGD SwissProt Entrez-Gene
Orthologous residues
TBX21 (human): S503‑p, TBX21 (mouse): S498‑p
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  GM03317, Jurkat (T lymphocyte), MEF (fibroblast), MSTO-211H (mesothelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) genetic knockout/knockin of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester, ionomycin increase

S507-p - TBX21 (human)
Modsite: RRVsPYPsSGDSsSP SwissProt Entrez-Gene
Orthologous residues
TBX21 (human): S507‑p, TBX21 (mouse): S502‑p
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  GM03317, Jurkat (T lymphocyte), MEF (fibroblast), MSTO-211H (mesothelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) genetic knockout/knockin of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester, ionomycin increase

S498-p - TBX21 (mouse)
Modsite: DTKRRRIsPYPsSGD SwissProt Entrez-Gene
Orthologous residues
TBX21 (human): S503‑p, TBX21 (mouse): S498‑p
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  GM03317, Jurkat (T lymphocyte), MEF (fibroblast), MSTO-211H (mesothelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse

S502-p - TBX21 (mouse)
Modsite: RRIsPYPsSGDSSsP SwissProt Entrez-Gene
Orthologous residues
TBX21 (human): S507‑p, TBX21 (mouse): S502‑p
Characterization
Methods used to characterize site in vivo flow cytometry, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  leukemia, T cell leukemia
Relevant cell lines - cell types - tissues:  GM03317, Jurkat (T lymphocyte), MEF (fibroblast), MSTO-211H (mesothelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse