Curated Information
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Home > Curated Information Page > PubMed Id: 18635821
Zhou Z, et al. (2008) Protein kinase G phosphorylates soluble guanylyl cyclase on serine 64 and inhibits its activity. Arterioscler Thromb Vasc Biol 28, 1803-10 18635821
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S64-p - GUCY1A1 (rat)
Modsite: SHPQRKTsRNRVYLH SwissProt Entrez-Gene
Orthologous residues
GUCY1A1 (human): S65‑p, GUCY1A1 (mouse): S65‑p, GUCY1A1 (rat): S64‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  A7r5 ('muscle, smooth'), aorta, COSm6 (renal), lung
Cellular systems studied:  cell lines, tissue
Species studied:  green monkey, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKG1 (human) inhibition of upstream enzyme, competition with site-specific peptide, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bradykinin increase
L-NAME bradykinin inhibit treatment-induced increase
SNP increase
peptide_agonist increase
peptide_agonist SNP augment treatment-induced increase
peptide_antagonist decrease
peptide_antagonist, SNP peptide_antagonist inhibit treatment-induced decrease
Rp-8pCPT-cGMPS decrease
8pCPT-cGMP increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited, phosphorylation
Comments:  downregulates phosphorylation of VASP S239

S236-p - VASP (rat)
Modsite: GAKLRKVsKEEASGG SwissProt Entrez-Gene
Orthologous residues
VASP (human): S239‑p, VASP (mouse): S235‑p, VASP (rat): S236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  A7r5 ('muscle, smooth')
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SNP increase